Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structures of asymmetric particles of tick-borne encephalitis virus provide insight into flavivirus assembly and maturation.

Science advances·2026
Same author

Allosteric disordering of eIF2B regulates the integrated stress response.

Nature chemical biology·2026
Same author

Structure determination and dual targeting of a plant TACO1 identifies its ancient role as an organelle translation regulator.

bioRxiv : the preprint server for biology·2026
Same author

Atomic resolution cryo-EM at 200 keV.

IUCrJ·2026
Same author

Diversity-driven biochemical survey reveals widespread dimerization throughout the rubisco superfamily.

Nature communications·2026
Same author

Twelve phosphomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments.

eLife·2026
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
查看所有相关文章

相关实验视频

Updated: Jul 3, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.1K

关于冷EM数据存档和验证的社区建议.

Gerard J Kleywegt1, Paul D Adams2, Sarah J Butcher3

  • 1EMBL-EBI, Cambridge, United Kingdom.

IUCrJ
|February 15, 2024
PubMed
概括
此摘要是机器生成的。

专家们在会议上确定了冷电子显微镜 (cryoEM) 结构的数据标准,重点是单粒子分析. 提出了关于存储,验证和存档的建议,以提高数据质量和可访问性.

关键词:
电子显微镜数据库电子显微镜数据库蛋白质数据库 蛋白质数据库生物信息学是一种生物信息学.化样本电子显微镜电子显微镜数据存档数据的存档.数据库就是数据库.质量控制质量控制质量控制一个粒子的冷EMEM.结构的确定 结构的确定验证验证的时间

更多相关视频

gP2S, an Information Management System for CryoEM Experiments
13:01

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

5.4K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

13.2K

相关实验视频

Last Updated: Jul 3, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.1K
gP2S, an Information Management System for CryoEM Experiments
13:01

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

5.4K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

13.2K

科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 数据科学数据科学数据科学

背景情况:

  • 电子显微镜 (cryoEM) 是一种强大的技术,用于确定生物大分子的3D结构.
  • 标准化数据要求对于可靠的冷EM结构的沉积,验证和存档至关重要.
  • 之前的努力突出了关于冷EM数据管理最佳实践的社区共识的需要.

研究的目的:

  • 汇集专家讨论和定义用于冷EM结构沉积和验证的数据要求.
  • 专注于单粒子分析 (SPA) 数据,这是一个广泛使用的冷EM方法.
  • 为改善现场数据质量,可复制性和可访问性制定共识建议.

主要方法:

  • 在EMBL-EBI召开了一次研讨会,与来自相关领域的47名专家 (数据处理,模型构建,改进,验证,存档) 合作.
  • 促进了关于冷EM数据管理当前挑战和未来需求的讨论.
  • 记录共识建议和确定未来方法开发的领域.

主要成果:

  • 产生了一系列关于冷EM数据存储和验证的共识建议,特别是对于SPA.
  • 确定了关键的挑战和需要进一步发展的领域在冷EM数据处理和存档.
  • 报告了研讨会的一些建议的初步实施情况.

结论:

  • 研讨会成功地就冷EM结构的基本数据要求达成共识.
  • 这些建议旨在提高科学界中结构数据的质量,完整性和可用性.
  • 需要在方法开发和实施方面持续努力,才能充分实现标准化数据实践的好处.