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在Pseudomonas koreensis MME3中氧化合成酶表达改善了植物生长促进特征

María M Labarthe^1,2, Guillermo A Maroniche^1,2, Lorenzo Lamattina^2,3

¹Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Buenos Aires, Argentina.

Applied microbiology and biotechnology

|February 15, 2024

概括

基因工程细菌Pseudomonas koreensis MME3表达Synechococcus氧化合成酶 (SyNOS) 增强了植物生长和使用效率,特别是在低条件下.

科学领域:

微生物学微生物学
植物科学植物科学
生物技术是生物技术.

背景情况:

可持续农业旨在通过提高 (N) 的利用来优化作物产量.
生物输入,如植物刺激剂和生物肥料,为提高的使用提供了有希望的解决方案.
对促进植物生长的草原细菌 (PGPR) 的基因改造可以提高它们的有效性.

研究的目的:

将Pseudomonas koreensis MME3进行基因工程,以表达Synechococcus氧化合成酶 (SyNOS).
评估重组菌株 (MME3:SyNOS) 对Brachypodium distachyon生长和吸收的影响.
评估SyNOS表达在改善植物适应缺乏的能力方面的有效性.

主要方法:

在P. koreensis MME3中使用miniTn7系统的synos基因的构成表达.
评估MME3:SyNOS生长,运动和生物膜形成.
用MME3:SyNOS和控制菌株在不同的可用性下对Brachypodium distachyon进行注射.

主要成果:

再组合的MME3:SyNOS菌株表现出改善的生长,运动性和生物膜形成.
用MME3:SyNOS接种的植物显示显著更高的根干重.
无论是MME3:SyNOS还是对照菌株,都改善了低N土壤中的吸收和使用效率.

结论:

SyNOS表达是一种可行的策略,可以增强根茎细菌的植物刺激能力.
改造的MME3:SyNOS菌株改善了植物根生长和使用效率.
这种方法提供了一个有前途的途径,以提高在有限的环境中的作物弹性.

关键词:

布拉基波迪乌姆 (Brachypodium) 是一个形体.氧化合成酶是一种氧化合成酶.气气是一种气.植物生长促进树枝细菌的生长.伪omonas 是一个类型的.

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