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通过L-阿斯巴拉金合成酶和多酸盐激酶结合转化和ATP再生提高L-阿斯巴拉金生物生产效率

Zijia Wei¹, Yuhua Zhang¹, Xuguo Duan²

¹College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.

Applied biochemistry and biotechnology

|February 15, 2024

概括

这项研究开发了一种有效的生物制备方法L-阿斯巴拉金,降低ATP成本. 优化的双酶系统显著提高了L-青素的产量和转化率,用于食品和医疗应用.

科学领域:

生物技术是生物技术.
酶工程是什么? 酶工程是什么?
生物化学合成生物化学合成

背景情况:

化学合成L-阿斯巴拉金面临着污染和副作用的挑战.
生物转化提供了效率,但由于ATP补充,造成了高成本.

研究的目的:

为了克服L-青素生物制备方面的挑战.
开发一种成本效益和高效的酶合成方法,用于L-阿斯巴拉金.

主要方法:

评估了来自大肠杆菌和L. salivarius. 的复合L-阿斯巴拉金合成酶.
来自Erysipelotrichaceae细菌的多酸盐激酶的重组表达.
建立了一种具有ATP再生的双酶合转化系统.

主要成果:

达到L-氨酸的最大产量为11.67g/L,转化率为88.43%.
演示了转换效率增加了5.03倍.
使用有效的ATP再生系统,降低了最初的ATP需求到5.66%.

结论:

开发的双酶系统显著提高了L-阿斯巴拉金制备的效率.
ATP再生系统有效降低了生产成本.
这种方法对食品和医药领域的工业L-黄素生产具有前景.

关键词:

在体外进行ATP再生.在L-阿斯巴拉基因.对于L-阿斯巴拉金合成酶来说,聚酸激酶是一种多酸激酶.

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