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Updated: Jul 3, 2025

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快速同热检测致病性Clostridioides difficile使用重组酶聚合酶放大

Iris Bachmann1, Ole Behrmann1, Marcel Klingenberg-Ernst2

  • 1Institute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, Universitätsplatz 1, 01968 Senftenberg, Germany.

Analytical chemistry
|February 15, 2024
PubMed
概括

快速诊断测试对于Clostridioides difficile感染 (CDI) 是至关重要的. 新的复合聚合酶放大 (RPA) 试验可以在短短15分钟内检测出关键毒素A和B,从而实现更快的治疗.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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