类似于真核细胞的Vps23的起源塑造了阿斯加德古生物中的ESCRT和ubiquitin系统之间的古老的功能相互作用
Zhongyi Lu1, Siyu Zhang1, Yang Liu1
1Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Cell reports
|February 15, 2024
概括
研究人员在阿斯加德古生物群中发现了ESCRT (运输所需的内体组分复合体) 和ubiquitin系统之间的古老相互作用. 这一发现揭示了真核细胞细胞过程的进化起源.
科学领域:
- 细胞生物学 细胞生物学
- 进化生物学 进化生物学
- 生物化学 生物化学
背景情况:
- 运输所需的内体分类复合体 (ESCRT) 和泛素系统之间的功能相互作用对于真核内膜系统的货物分类至关重要.
- 这种ESCRT-ubiquitin系统相互作用的进化起源在很大程度上是未知的.
研究的目的:
- 调查ESCRT和ubiquitin系统相互作用的进化起源.
- 阐明UEV-Vps23蛋白家族在阿斯加德古生物中的作用.
主要方法:
- 生物化学试验用于研究蛋白质与ubiquitin的相互作用.
- 结构同质分析. 结构同质分析.
- 研究阿斯加德古生物的无处不在级联.
主要成果:
- 在阿斯加德古生物中确定了一个UEV-Vps23蛋白家族,它调解着古老的ESCRT-ubiquitin系统相互作用.
- 证明UEV域与ubiquitin结合,作为ubiquitin货物的传感器.
- 显示Vps23域的稳定盒通过阿斯加德E1,E2和RING E3级联经历了无处不在.
- 揭示了UEV-Vps23通过在自抑制和活性形式之间切换来调节相互作用.
- 在UEV-Vps23,真核体Vps23和古老的CdvA之间发现了共享的同质性,这表明了共同的进化起源.
结论:
- 阿斯加德古生物中的UEV-Vps23蛋白家族提供了关于古代ESCRT-ubiquitin系统相互作用的见解.
- 这种相互作用可能早于阿斯加德古生物和真核生物的分化.
- 这些发现扩大了我们对基本细胞分类机制进化的理解.
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