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在日本采集的两种相同基因型的抗宏素耐药性百日咳 Bordetella 隔离物的全基因组比较

Kentaro Koide¹, Yumi Uchitani², Takahiro Yamaguchi³

¹Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.

|February 15, 2024

概括

2018年,日本出现了耐宏杆菌百日咳 (MRBP) 菌株. 全基因组分析显示,这些菌株与中国MRBP密切相关,但在基因组上是不同的,这表明它们是分开引入的.

科学领域:

微生物学微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

耐宏杆菌百日咳 (MRBP) 构成了严重的公共卫生威胁,限制了百日咳的治疗选择.
在中国,MRBP已经变得普遍,在其他亚洲国家偶尔检测到.
2018年,两种MRBP临床菌株首次在日本被发现,流行病学联系不清楚.

研究的目的:

为了研究两种日本MRBP分离物之间的遗传关系.
确定日本MRBP的起源和传播途径.

主要方法:

两个日本MRBP分离物 (BP616和BP625) 的全基因组测序.
使用全基因组单核酸多态基因组基因组分析.
基因组结构比较以确定遗传变异.

主要成果:

这两种日本分离物共享相同的毒性相关基因和MT195基因型,类似于中国MRBP菌株.
遗传学分析将这些分离物置于中国MRBP类中,但它们之间发现了11个单核酸多态性差异.
基因组结构分析揭示了日本分离物之间的显著差异,包括两个大的反转和一个转换.

结论:

这两个日本MRBP分离物与中国菌株密切相关,但在基因组上是不同的.
这些发现表明,MRBP从中国大陆通过不同的传播途径进入日本的独立引入事件.

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