概括
光声扩展显微镜 (PA-ExM) 结合了光学分辨率光声扩展显微镜 (OR-PAM) 和扩展显微镜 (ExM) 进行增强的3D有机体成像. 这种新的技术可以实现细胞结构的高质量成像,而无需超高数值孔径的目标.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 细胞生物学 细胞生物学
- 光学和光子学 在光学和光子学.
背景情况:
- 光学分辨率光声学显微镜 (OR-PAM) 为可视化细胞器官提供高空间分辨率和光学对比度.
- 传统的OR-PAM依赖于高数值光圈 (NA) 目标和高频超声波探测器进行3D成像.
- 扩展显微镜 (ExM) 通过同位素扩大细胞来实现纳米分辨率,绕过了超高NA目标的需要.
研究的目的:
- 开发和评估光声扩展显微镜 (PA-ExM),一种混合成像技术.
- 结合OR-PAM和ExM的优势,实现先进的3D器官成像.
- 为了证明在近红外光下对有机细胞的无标签成像.
主要方法:
- 发展光声扩展显微镜 (PA-ExM).
- 使用近红外光进行成像.
- 使用无标签黑色素瘤细胞进行评估.
- 使用扩展显微镜和不使用扩展显微镜的成像性能比较.
主要成果:
- PA-ExM成功地对细胞的3D微观结构进行了成像.
- 使用40×镜头在扩展细胞中的黑色素体分布的图像质量与使用100×油浸镜头成像的未扩展细胞相似.
- 在黑色素瘤细胞中证明了黑色素体的无标签成像.
结论:
- PA-ExM集成了OR-PAM和ExM的优势,用于有效的3D器官成像.
- 该技术提供了高质量的成像,与传统的高NA方法相比,但放大目标较低.
- PA-ExM显示出研究细胞器官的巨大潜力.
相关概念视频
Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Electron Microscopy
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Transmission Electron Microscopy
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Microscopy Techniques
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...


