使用HVTEM和UHVTEM在植物染色体上定位和定量分布染色体结构蛋白Topoisomerase II

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Channarong Sartsanga¹, Rinyaporn Phengchat², Toshiyuki Wako³

¹Graduate School of Human Development and Environment, Kobe University, Tsurukabuto 3-11, Nada-ku, 657-8501, Kobe, Japan.

Micron (Oxford, England : 1993)

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概括

拓酶II (TopoII) 对于植物染色体结构至关重要. 这项研究揭示了TopoII.

科学领域:

植物分子生物学植物分子生物学
染色体的结构和动态.
细胞生物学细胞生物学

背景情况:

拓酶II (TopoII) 对于基因相染色体结构和紧缩至关重要.
它在植物染色体中的精确定位在很大程度上仍然未被描述.
之前的研究对植物系统中的TopoII分布提供了有限的见解.

研究的目的:

为了研究大麦染色体中Topoisomerase II的局部化,对齐和密度.
评估先进显微镜技术对可视化单个TopoII分子的适用性.
阐明TopoII在大麦染色体组织中的作用.

主要方法:

使用高压传输电子显微镜 (HVTEM) 和超高压传输电子显微镜 (UHVTEM).
在1.4纳米尺度上使用免疫黄金标签和银色增强用于TopoII可视化.
在大麦染色体中量化TopoII分子的分布和密度.

主要成果:

具有免疫黄金标签的HVTEM和UHVTEM可以有效地检测像TopoII.这样的结构蛋白.
在大麦染色体上绘制了超过31,000个TopoII分子.
TopoII在染色体臂上表现出不特定的模式,但在核细胞组织者区域 (NORs) 和中心体积积累.
在终端区域,NOR和中心区域观察到TopoII密度增加.

结论:

这项研究成功地将TopoII在大麦染色体中的分子水平可视化.
这些发现支持TopoII在维护染色体结构和组织方面的已确定的功能.
采用的方法适用于详细分析植物染色体中的蛋白质定位.

关键词:

染色体结构蛋白质是染色体的结构蛋白.高压传输电子显微镜电子显微镜植物染色体是什么植物染色体这里有多么多.超高压传输电子显微镜超高压传输电子显微镜

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