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相关概念视频

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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相关实验视频

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Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
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通过4D-STEM和EELS进行定量原子截面分析.

Shahar Seifer1, Lothar Houben2, Michael Elbaum1

  • 1Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel.

Ultramicroscopy
|February 15, 2024
PubMed
概括
此摘要是机器生成的。

本研究使用四维扫描传输电子显微镜 (4D-STEM) 来确定无形材料中的原子截面信息. 引入了新的参数,Zeta和eta,用于生物标本中的材料识别.

关键词:
4D STEM 是一个 4D STEM.贝特的理论就是理论.不弹性的横截面.子散射是因为等离子散射.Z-对比传输电子显微镜

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科学领域:

  • 材料科学 材料科学 材料科学
  • 分析化学 分析化学
  • 物理 物理学 物理

背景情况:

  • 无形材料缺乏远程原子顺序,这使得传统的衍射方法对结构分析具有挑战性.
  • 从无形标本中提取原子级信息对于理解它们的特性和组成至关重要.

研究的目的:

  • 为了证明四维扫描传输电子显微镜 (4D-STEM) 在无形材料中量化原子截面信息的实用性.
  • 使用电子显微镜在无形样本中开发材料识别的新参数.

主要方法:

  • 利用4D-STEM用200keV电子记录无形碳,,无形冰和玻璃化酸盐缓冲溶液的衍射模式.
  • 获得的电子能量损失光谱 (EELS) 数据在各种厚度和能量.
  • 采用卷积模型来解混合弹性和不弹性散射贡献.

主要成果:

  • 对等离子激发的测量差异截面遵循1/θ2分布,与离散二极转换的理论计算相匹配.
  • 在较高的角度,弹性散射遵循1/θ3·7的依赖性,与轻元素的原子数线性相关.
  • 引入了Zeta (弹性-不弹性散射比) 和eta (弹性系数除以厚度) 参数用于材料特性.

结论:

  • 4D-STEM与EELS相结合,可以有效地从无形材料中提取原子截面数据.
  • 开发的Zeta和eta参数为识别无形有机和生物标本中的材料类提供了一种手段.
  • 这些发现为复杂无形系统的详细组成分析提供了一条途径.