吸附带喷雾双重质谱仪使用涂有混合模式微粒的片进行测量
Carlos Calero-Cañuelo1, Francisco Antonio Casado-Carmona2, Rafael Lucena1
1Affordable and Sustainable Sample Preparation (AS(2)P) Research Group, Departamento de Química Analítica, Instituto Químico para la Energía y el Medioambiente IQUEMA, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071, Córdoba, Spain.
Talanta
|February 15, 2024
概括
这项研究引入了吸附带-喷雾双重质谱法 (STS-MS/MS),这是一种结合样本准备和分析的新方法. 这种技术通过克服矩阵效应和提高生物流体中化合物确定量的吞吐量来增强生物分析.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 法医科学 法医科学 法医科学
背景情况:
- 直接质谱 (MS) 提供了快速的生物分析,但受到矩阵效应和低分析物度的限制.
- 有效的样本准备对于减轻生物流体分析中的这些局限性至关重要.
研究的目的:
- 开发和评估一种新的吸附带喷雾联质谱法 (STS-MS/MS) 技术.
- 将样品制备和MS分析结合到一个单一,高效的生物分析设备中.
- 通过在唾液中确定可代因来证明该方法的实用性.
主要方法:
- 在吸附带 (ST) 上使用混合模式的聚合物微粒来分离分析物和MS/MS分析.
- 采用多变量方法来优化提取变量.
- 在唾液样本中验证了可代因测定方法.
主要成果:
- 达到了0.3微克L-1的可检测极限.
- 证明了高精度 (<9.4% RSD) 和准确性 (78-98%的恢复).
- 通过将其与商业液体染色学-MS系统相结合,展示了ST的多功能性.
结论:
- STS-MS/MS提供了一种负担得起和高效的生物分析策略,克服了共同的挑战.
- 吸附带技术提高了样品吞吐量,可以集成到现有的分析工作流中.
- 这种方法在各种生物分析场景中为快速决策提供了有价值的工具.
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