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Updated: Aug 5, 2026

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在活单细胞成像试验中监测Chk1激酶活性动态

Vivianne Lebrec1, Olivier Gavet2

  • 1Division of Cancer Biology, The Institute of Cancer Research, London, United Kingdom.

Methods in cell biology
|February 15, 2024
PubMed
概括

该ATR/Chk1通路调节细胞周期进展和DNA修复. 一个新的FRET报告器允许在正常生长和DNA损伤反应期间精确监测活细胞中的Chk1激酶活性.

关键词:
在 Chk1 的位置上,我们可以看到 Chk1 .对DNA损伤的反应反应在FRET传感器上.激酶活动的激酶活性.活细胞成像 活细胞成像复制应激是复制应激的一种.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...