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原生鱼:一种低和高通量试验,用于分析端粒 (ALT) 途径的替代延长

Benura Azeroglu¹, Laurent Ozbun², Gianluca Pegoraro²

¹Laboratory of Genome Integrity, National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, United States.

Methods in cell biology

|February 15, 2024

概括

端粒的替代延长 (ALT) 是癌症的一个标志. 我们开发了Native-FISH (N-FISH),这是一种用于检测ALT阳性细胞的新型试验,可用于单细胞分析和针对向癌症治疗的高通量查.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

替代端粒延长 (ALT) 是一种端粒维持机制,约15%的人类癌症使用.
阳性ALT癌症依赖于重组,提供独特的治疗点.
目前的ALT检测测定缺乏单细胞分辨率和高通量查能力.

研究的目的:

开发一种可扩展和灵敏的测试方法来检测ALT阳性癌细胞.
克服现有方法的局限性,比如C圆试验 (CCA).
为了能够识别新的ALT调节剂和治疗点.

主要方法:

开发和验证Native-FISH (N-FISH) 用于可视化单链端粒DNA.
N-FISH允许在单细胞中直接可视化ALT特异性DNA结构.
详细的协议适用于低吞吐量 (蜂线) 和高吞吐量 (小型化384井格式) 应用.

主要成果:

N-FISH提供关于ALT状态的单细胞数据.
该测试适用于没有DNA隔离或放大的固定组织.
对于高通量查 (HTS),N-FISH可以缩小.

结论:

N-FISH是一种多功能且可扩展的ALT检测方法.
小型化的N-FISH与HTS相结合可以加速ALT调节者的发现.
这种测定有助于开发针对ALT阳性癌症的向疗法.

关键词:

这就是ALT ALT.高内容成像高内容成像高通量成像高通量成像本地鱼类 - - 本地鱼类泰洛米尔 (Telomere) 是一种电离体.单细胞测定试验单细胞测定

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