相关实验视频

Updated: Jul 15, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

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一种高含量流细胞计和基于双CRISPR-Cas9的平台,用于量化遗传相互作用

Natasha Ramakrishnan1, Taylor Malachowski1, Priyanka Verma1

  • 1Division of Oncology, Department of Medicine, Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, United States.

Methods in cell biology
|February 15, 2024
PubMed
概括

这项研究引入了一种基于流量的新型CRISPR屏幕来研究基因相互作用. 该方法克服了传统选器的局限性,使得基因相互作用及其对细胞活力随时间推移的影响能够得到精确的分析.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?修复DNA的修复DNA的修复竞争 GFP 竞争 GFP 的竞争高含量流动细胞计学 高含量流动细胞计学复制复制复制复制复制复制复制这就是SaCas9的原因.

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...