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通过牛津纳米孔测序来增强trypanosomatid识别和基因定型:开发和验证基于18SrRNA amplicon的方法
Lissa Cruz-Saavedra1, Carlos Ospina1, Luz H Patiño1
1Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
The Journal of molecular diagnostics : JMD
|February 15, 2024
概括
使用牛津纳米孔技术的新的基于片的测序 (ABS) 方法改善了Trypanosomatid检测和基因类型. 这种敏感的工具在各种样本中准确识别多种物种和菌株,有助于疾病控制.
科学领域:
- 寄生虫学的寄生虫学
- 基因组学就是基因组学.
- 分子诊断学 分子诊断
背景情况:
- 松胺类药物会引起严重的医疗和兽医问题.
- 目前的诊断和基因定型方法复杂且多步骤.
- 准确的检测和区分对于疾病管理至关重要.
研究的目的:
- 开发一种基于AMPLICON的测序 (ABS) 方法,以提高Trypanosomatid的检测和基因定型.
- 使用牛津纳米孔长读序列来提高分辨率.
- 针对保存的18S rDNA基因,以实现广泛的应用.
主要方法:
- 开发一种基于片的测序 (ABS) 协议.
- 牛津纳米孔长读测序的应用.
- 针对 18S rDNA 基因进行 Trypanosomatid 鉴定.
主要成果:
- 通过ABS方法准确区分了11个Trypanosoma物种和6个Trypanosoma cruzi类型单元.
- 观察到与传统方法的高度一致性 (κ=0.729).
- 该方法检测了并发感染,并且在人类,动物和载体样本中具有低检测极限 (1寄生虫/毫升).
结论:
- 松胺-ABS方法提供了高分辨率,灵敏度和精度.
- 这是一个有价值的工具,以了解Trypanosomatid的流行病学和动态.
- 这种方法可以加强疾病控制策略和有针对性的干预措施.
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