在真核生物中的结构多样性 光合作用 光采集光
Masakazu Iwai1,2, Dhruv Patel-Tupper2, Krishna K Niyogi1,2,3
1Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA;
Annual review of plant biology
|February 15, 2024
概括
光合作用生物利用多种不同的光收获复合体 (LHC) 蛋白质来生存. 结晶电子显微镜结构的比较揭示了LHC中的进化模式,有助于作物改善.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 进化生物学 进化生物学
背景情况:
- 光合作用,利用阳光转化二氧化碳,已经存在超过35亿年.
- 光采集复合体 (LHC) 蛋白质对于光系统组装,能量转移和光保护在不同的环境中至关重要.
- 最近的结构研究提供了对LHC功能驱动的分子组件的高分辨率洞察.
研究的目的:
- 为了比较和对比光合作用真核生物中的LHC蛋白质的冷电子显微镜结构.
- 为了确定负责各种光采集策略的结构动图.
- 了解微小的结构变化如何导致大规模的寡头重组和功能多样化.
主要方法:
- 对最近发表的冷电子显微镜 (cryo-EM) 结构进行比较分析.
- 专注于光合作用的真核生物.
- 检查蛋白质结构,颜料-蛋白质相互作用和脂质-蛋白质相互作用.
主要成果:
- 在不同的LHC中识别保存和可变的结构图案.
- 观察微妙的单体变化如何影响LHC寡合化和功能的观察.
- 发现反复出现的模式,表明了功能多样化的进化途径.
结论:
- LHC蛋白质的结构多样性是各种光采集策略的基础.
- 了解LHC蛋白与环境的相互作用是提高作物生产率的关键.
- 对比结构分析为LHC功能多样化提供了进化见解.
相关概念视频
Photosystems
4.8K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
4.8K
The Antenna Complex
6.0K
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency...
6.0K
Photosystem I
62.2K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
62.2K
Photosystem II
70.4K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
70.4K
The Photochemical Reaction Center
4.1K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.1K
The Anatomy of Chloroplasts
5.1K
Green algae and plants, including green stems and unripe fruit, harbor specialized organelles called chloroplasts to carry out photosynthesis. They coordinate both stages of photosynthesis — the light-dependent reactions and the light-independent reactions. The light-dependent reactions use sunlight to release oxygen and produce chemical energy in the form of ATP and NADPH, and the light-independent reactions capture CO2 and use ATP and NADPH to produce sugar.
Structure of...
Structure of...
5.1K


