维生素D3对乳腺癌相关纤维细胞的双重作用
Natalia Łabędź1,2, Artur Anisiewicz3, Martyna Stachowicz-Suhs3
1Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Weigla 12, 53-114, Wroclaw, Poland. natalia.labedz@hirszfeld.pl.
BMC cancer
|February 15, 2024
概括
维生素D3 (calcifediol) 在乳腺癌中改变与癌症相关的纤维细胞 (CAF),可能减少瘤细胞迁移. 这些发现表明,二醇在调节瘤微环境方面起着作用.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- 癌症相关纤维细胞 (CAFs) 是瘤微环境中的关键参与者.
- 已知维生素D3 (VD3) 的体外抗瘤作用,但其对乳腺CAF的影响在很大程度上仍未被探索.
- 这项研究研究了利醇对从乳腺癌组织中分离出来的CAFs的ex vivo作用.
研究的目的:
- 分析卡尔西醇对乳腺癌相关纤维细胞 (CAFs) 的活体作用.
- 评估酸盐如何影响CAF表型,基因表达,蛋白质表达和分泌.
- 确定酸治疗的CAF对乳腺癌细胞迁移和蛋白质表达的影响.
主要方法:
- 在培养CAF时,使用1和10nM的酸.
- 评估了表型,基因表达,蛋白质表达和CAFs的分泌.
- 来自酸治疗的CAF的条件介质被用于评估对MCF-7和MDA-MB-231细胞迁移和蛋白质表达的影响.
主要成果:
- 酸降低了CAF活力,CCL2,MMP9,TNC表达和MMP-2活性,同时增加了PDPN,SPP1和TIMP1.
- 根据患者的维生素D3状态,更年期状态和更年期状态,效果有所不同.
- 接受CAF治疗的条件介质在非转移和绝经后的患者中减少了MCF-7细胞迁移.
结论:
- 酸在基因和蛋白质水平上对乳腺CAF产生复杂的影响,影响它们的亲或反瘤原生性质.
- CAFs的抗癌两极化可能由降低的CCL2,TNC,MMP9和MMP-2调解,与增加的PDPN,TIMP1和SPP1.1形成对比.
- 从非转移和绝经后患者的CAFs的ex vivo利醇治疗减少了乳腺癌细胞迁移,表明了治疗潜力.
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