在兴奋剂控制中SFC-MS的常规应用:使用三个不同的SFC-MS仪器分析3×1000个尿样
Ivana Gavrilović1, Bernhard Wüst2, Jonathan Danaceau3
1Drug Control Centre, King's Forensics, Department of Analytical, Environmental and Forensic Sciences, King's College London, London, UK.
Drug testing and analysis
|February 16, 2024
概括
超临界流体染色学-质谱学 (SFC-MS) 是反兴奋剂实验室的一个可行的工具. 这项研究证明了SFC-MS.
科学领域:
- 分析化学 分析化学
- 法医科学 法医科学 法医科学
- 生物化学 生物化学
背景情况:
- 超临界流体染色学-质谱学 (SFC-MS) 越来越多地因其分析能力而得到认可.
- 反兴奋剂社区正在探索用于运动员药物检测的新技术.
- 目前的反兴奋剂策略依赖于气体和液体染色学与质谱学相结合.
研究的目的:
- 评估SFC-MS在常规反兴奋剂控制中的适用性.
- 评估来自多个供应商的SFC-MS仪器的性能,以进行兴奋剂分析.
- 确定SFC-MS作为反兴奋剂实验室补充技术的适用性.
主要方法:
- 使用SFC-MS.分析大约3000个相同的反兴奋剂样本.
- 使用了来自Agilent Technologies,Waters公司和Shimadzu公司的SFC-MS仪器.
- 采用"稀释和注射"的方法,包括或不包括葡萄糖化物代谢物的水解.
主要成果:
- 大多数化合物表现出极好的染色学分离.
- 由于与内源干扰的共,一些分析物需要质谱 (MS) 歧视.
- 观察到稳定的保留时间 (CV ≤0.5%) 和良好的,对称的峰值形状.
- 在反兴奋剂测试中,所有三个测试的SFC-MS仪器设置都被证明适合目的.
结论:
- SFC-MS是一种灵敏,可复制和强大的分析技术.
- SFC-MS已准备好整合到常规的反兴奋剂实验室工作流程中.
- SFC-MS可以有效地补充现有的染色学和质谱法用于兴奋剂控制.
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