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Omid Karami¹, Azadeh Khadem¹, Arezoo Rahimi¹

¹Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, Netherlands.

The Plant journal : for cell and molecular biology

|February 16, 2024

概括

抑制天然auxin出口可以增强植物再生系统,如体质胚胎生成 (SE) 和新芽再生. 与合成辅酶相比,这种方法提高了效率和幼苗的发展.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
发育生物学是发展生物学.

背景情况:

高效的体外植物再生对于作物育种和基因组编辑至关重要.
身体胚胎发生 (SE) 和新发芽再生通常依赖于合成辅酶,如2,4-D.
由于植物细胞的快速流出,自然辅酶在诱导再生方面通常效果较差.

研究的目的:

调查是否抑制内源性auxin流量可以使天然auxins成为植物再生的有效诱导剂.
为了比较使用排放抑制天然辅酶与合成辅酶的再生效率和结果.

主要方法:

用天然辅酶和辅酶运输抑制剂 (例如,纳甲酸 - NPA) 治疗阿拉比多普西斯不成熟的胚胎 (IZE).
在auxin流量突变体中SE效率的分析 (pin2,abcb1 abcb19).
在胡卜悬浮细胞中诱导SE,并在Brassica napus中使用阻断流量辅酶进行de novo发芽再生.

主要成果:

使用NPA暂时抑制auxin流量使天然auxins能够有效地诱导Arabidopsis IZEs中的SE.
在IAA治疗后,auxin流量突变体显示SE效率提高.
与2,4-D相比,使用阻断排放的天然辅酶进行再生,导致更高的转化率和更同步的发育.
在胡卜细胞中诱导了SE,而在Brassica napus中改善了发芽再生的4-Cl-IAA.

结论:

素排放显著限制了天然素在植物再生中的效率.
抑制奥克辛流量将天然奥克辛转化为SE和de novo芽再生的强大和有效剂.
这种方法为合成辅酶提供了一个有希望的替代品,改善了再生结果和植物发育统一性.

关键词:

2,4-二二氧乙酸的使用.阿拉比多普西斯 (Arabidopsis) 是一种植物.这就是Brassica Brassica.抑制奥克辛的运输抑制胡卜胡卜是一种印醇-3-乙酸 (IAA) 是一种射出再生再生的火箭.实体胚胎发生是体质胚胎发生.

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