一个RNAi效率相关核酶 (REase) 的功能性表征和假定调节机制在秋季军,Spodoptera frugiperda
Xiaoyang Zhou1, Jiaping Wei1, Huichen Ge1
1College of Plant Protection, Yangzhou University, Yangzhou 225009, China.
Journal of agricultural and food chemistry
|February 16, 2024
概括
我们确定了Spodoptera frugiperda REase (SfREase),并发现它会降解双链RNA (dsRNA). 它由FOXO进行的调节为类昆虫的RNAi害虫控制提供了新的策略.
科学领域:
- 分子生物学分子生物学
- 昆虫的生物化学 昆虫的生物化学
- 在RNA干扰过程中,RNA干扰
背景情况:
- 类动物特异性RNAi效率相关核酶 (REase) 降解双链RNA (dsRNA).
- 昆虫中REase的调节机制在很大程度上仍未被描述.
研究的目的:
- 为了识别和描述SfREase在Spodoptera frugiperda中的特征.
- 阐明SfREase在RNAi效率中的调节机制和功能作用.
主要方法:
- 在Spodoptera frugiperda中的SfREase的识别和特征.
- 在不同条件下的SfREase表达的分析 (dsEGFP,高温,饥饿).
- 使用重组SfREase进行体外降解试验,并分析幼虫液体中的dsRNA降解.
- 通过RNA干扰 (RNAi) 和双化酶记者测定来确定FOXO.的SfREase调节.
主要成果:
- SfREase表达通过dseGFP和高温进行上调,并通过饥饿进行下调.
- Knockdown 的 SfREase 显著减少 dsRNA 降解,并提高 RNAi 的效率.
- 再组合SfREase表现出度依赖的dsRNA降解活性.
- FOXO被确定为SfREase活动的负调节剂.
结论:
- 在Spodoptera frugiperda中,sfREase在dsRNA降解中起着至关重要的作用,影响RNAi的效率.
- 这项研究揭示了涉及FOXO的新型监管机制,为REase功能提供了洞察力.
- 这些发现对开发针对性RNAi基于Spodoptera frugiperda的害虫控制策略具有重大意义.
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