相关实验视频

Updated: Jul 3, 2025

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

在培养肝细胞中与SARS-CoV-2相关的复制和损伤

Suman Pradhan¹, Susan D Rouster², Jason T Blackard²

¹Department of Molecular and Cellular Biosciences, University of Cincinnati College of Medicine, Cincinnati, Ohio.

Pathogens & immunity

|February 16, 2024

概括

SARS-CoV-2 在肝细胞中活跃复制,通过亡导致细胞死亡. 抗病毒药物remdesivir有效地阻止了这种病毒复制和肝损伤,这表明活跃复制有助于COVID-19中的肝功能障碍.

科学领域:

肝病学肝病学是一种肝病学.
病毒学病毒学
细胞生物学细胞生物学

背景情况:

肝功能障碍是SARS-CoV-2感染的常见特征.
关于SARS-CoV-2引起的肝损伤的确切机制仍在争论中,关于病毒复制与非细胞病变影响的讨论正在进行中.
这项研究调查了SARS-CoV-2在肝细胞中的复制及其在肝损伤中的作用.

研究的目的:

为了评估SARS-CoV-2在人类肝细胞系和初级肝细胞中的复制.
为了确定SARS-CoV-2感染是否通过亡途径诱导肝细胞损伤.
评估抗病毒药物remdesivir在缓解SARS-CoV-2复制和相关肝细胞损伤方面的有效性.

主要方法:

永久性肝细胞细胞系 (HepG2,Huh7.5) 和人类初级肝细胞暴露于SARS-CoV-2.
使用斑块测定和针对病毒尖端蛋白的免疫组织化学染色来评估病毒复制.
通过caspase-3表达的亡的评估,有或没有雷梅西维尔治疗.

主要成果:

肝细胞表现出活跃的SARS-CoV-2复制.
观察到显著的细胞病变效应和亡途径 (caspase-3) 的激活.
雷梅西维尔剂量依赖地抑制病毒感染,并没有导致独立的肝细胞损伤.

结论:

肝细胞对SARS-CoV-2复制非常敏感,通过亡导致细胞快速死亡.
雷梅西维尔有效地废除了病毒复制和随后的肝细胞损伤.
活跃的SARS-CoV-2复制可能是COVID-19患者肝酶异常的主要原因.

关键词:

在这里,我们可以看到COVID COVID COVID.这就是SARS-CoV-2病毒.灭症 (apoptosis) 是一种死亡的过程.肝细胞肝细胞肝细胞肝脏肝脏肝脏肝脏肝脏肝脏雷梅西维尔 (remdesivir) 是一种药物.复制复制复制复制复制复制复制

更多相关视频

A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Published on: March 10, 2017

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...