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相关概念视频

Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
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Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
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相关实验视频

Updated: Jul 3, 2025

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
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高压巴氏杀菌:同时进行原生组织脱细胞化和灭菌.

Akihisa Otaka1, Takashi Yamamoto2, Tetsuji Yamaoka1

  • 1Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan.

Regenerative therapy
|February 16, 2024
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概括

高水静压化 (HHP) 通过使用重复循环和抗生素介质有效消毒脱细胞化异体移植. 这种优化的HHP过程对于组织衍生产品的临床应用至关重要.

关键词:
生物材料是一种生物材料.脱细胞化 脱细胞化高水静压的高水静压压力消毒灭菌 消毒灭菌 消毒灭菌异族人体移植 异族人体移植

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相关实验视频

Last Updated: Jul 3, 2025

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科学领域:

  • 生物材料科学 生物材料科学
  • 微生物学 微生物学
  • 消毒技术 消毒技术

背景情况:

  • 终端绝育对于脱细胞化异体移植的临床使用至关重要.
  • 高水位压缩 (HHP) 是异种移植脱细胞化和脱污染的潜在方法,但其灭菌效果尚不清楚.

研究的目的:

  • 调查HHP在36°C对抗抗性子形成细菌的消毒效果.
  • 评估重复压缩与持续压缩以及含有抗生素的介质对细菌消除的影响.

主要方法:

  • 形成子的细菌 (Bacillus atrophaeus,Geobacillus stearothermophilus) 在重复和持续的加压协议下接受了HHP.
  • 实验是在压缩介质中进行的,有或没有抗生素剂.

主要成果:

  • 持续加压,通常用于脱细胞化,显示细菌消除有限.
  • 重复加压显著提高了消毒的有效性.
  • 将抗生素添加到压缩介质中进一步改善了消毒,实现了杀菌水平.

结论:

  • 优化的HHP,利用重复加压和含有抗生素的介质,可以在脱细胞化过程中有效消毒生物组织.
  • 这种方法为生产无菌组织衍生的医疗保健产品提供了一个有前途的战略.