相关实验视频
Updated: May 11, 2026

07:54
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
Published on: July 25, 2017
14.3K
使用比较基因组分析对五种候选益生菌乳酸菌的安全性评估
Patrick Josemaria D R Altavas1, Mia Beatriz C Amoranto1, Sang Hoon Kim2
1Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Ermita, Manila 1000, Philippines.
Access microbiology
|February 16, 2024
概括
益生菌细菌的安全性通过分析病毒性因素的基因组来评估. 虽然没有发现抗生素耐药性基因,但一些菌株具有与生存和粘附相关的基因,这种基因在病原体中很常见.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 益生菌安全性 益生菌安全性
背景情况:
- 乳杆菌属复杂益生菌通常是安全的,但可以是致病的.
- 了解遗传因素对于评估益生菌安全至关重要.
- 特定的益生菌菌株,如Limosilactobacillus mucosae LM1和Lactiplantibacillus plantarum SK151,需要进行安全评估.
研究的目的:
- 为了确定选定的益生菌菌株中的潜在毒性因素和抗生素耐药性 (AR) 基因.
- 为了评估Limosilactobacillus mucosae LM1,Lactiplantibacillus plantarum SK151,Lactiplantibacillus plantarum BS25,Limosilactobacillus fermentum SK152和Lactobacillus johnsonii PF01.01的安全性概况,该研究的目标是:
- 为益生菌的临床前安全评估策略做出贡献.
主要方法:
- 用L. plantarum BS25进行基因组测序,并使用RAST进行注释.
- 使用命令行BLAST对抗病毒性因素数据库 (VFDB) 和综合抗生素耐药性数据库 (CARD) 的生物信息分析.
- 使用ResFinder检测获得的AR基因.
主要成果:
- 确定了与细菌生存,血小板聚合,表面粘附,生物膜形成和免疫调节相关的基因.
- 使用CARD和ResFinder没有检测到获得的抗生素耐药性基因.
- 分析的益生菌菌株具有与致病性细菌中发现的基因相同的基因,主要与生存和坚持有关.
结论:
- 这项研究提供了对益生菌潜在致病性的遗传基础的见解.
- 全基因组数据分析是临床前益生菌安全性评估的宝贵工具.
- 基因挖掘与实验验证相结合,可以提高未来的益生菌安全策略.
相关概念视频
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Applications of Molecular Taxonomy
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...

