在单个动物宿主血统中对初始的图拉汉塔病毒物种进行全基因组支持
Anton Labutin1, Gerald Heckel1
1Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, Bern 3012, Switzerland.
Virus evolution
|February 16, 2024
概括
在常见飞中,两个图拉整体病毒 (TULV) 基群表明初始的病毒物种化. 这些独特的病毒系可能在受宿主遗传的影响下演变为新物种.
科学领域:
- 病毒学 病毒学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 病毒进化分歧通常与宿主共同分歧或宿主转移有关.
- 病毒物种中分歧的基因组分类 (功能等价物与初始物种) 的状态基本上是未知的.
- Tula orthohantavirus (TULV) 在常见的飞鸟 (Microtus arvalis) 中提供了一个研究病毒演变的模型.
研究的目的:
- 调查不同的TULV菌群是否代表高进化速率或初始病毒物种的功能等价的副产品.
- 分析来自TULV细胞群的基因组数据,这些细胞群属于它们自然宿主的单一进化血统.
主要方法:
- 使用逆转录 (RT) -PCR.感染来自TULV分类接触区域的42个地点的小鼠.
- 测序了23个TULV中央北基因组和21个TULV中央南基因组.
- 分析核酸和氨基酸差异,重组,重新排序和地理位置的差异.
主要成果:
- 测序的TULV基因组显示了显著的核酸 (14.9-18.5%) 和氨基酸 (2.2-3.7%) 差异,没有重组.
- 突如其来的 (<1公里) 在连续宿主景观中的TULV片之间发生的parapatric过渡.
- 宿主基因组分化在TULV过渡过程中比在宿主混合区中弱.
结论:
- 帕帕特里克的TULV群可能代表功能上不同的,初始的物种.
- 刚开始的TULV物种可能会受到宿主遗传多态化的差异影响.
- 自然病毒接触区对于研究RNA病毒传播动态非常有价值.
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