Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cholinesterases: Distribution and Function01:22

Cholinesterases: Distribution and Function

461
Cholinesterases are a group of serine hydrolase enzymes that play a crucial role in the breakdown of choline esters. The two primary types of cholinesterases are acetylcholinesterases (AChEs) and butyrylcholinesterase (BuChEs), which differ in their distribution, function, and substrate specificity. AChEs, also known as true cholinesterases, specifically hydrolyze acetylcholine, while BuChEs, often referred to as pseudocholinesterases, can hydrolyze various choline esters, including...
461
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K
Introduction to Plant Diversity02:22

Introduction to Plant Diversity

44.7K
From Water to Land
44.7K
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

2.7K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.7K
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

3.6K
 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
3.6K
Plant Cell Wall02:43

Plant Cell Wall

56.6K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Metastable multimeric G-quadruplex 2'FY-RNA aptamers that selectively bind pyoverdines.

Scientific reports·2026
Same author

Systems-level proteomic models of cotton fiber development: a high-resolution data resource to analyze cell dynamics and trait engineering.

Plant physiology·2026
Same author

Xyloglucan xylosyltransferase stem region mediates heterodimer formation.

Nature communications·2026
Same author

Interaction with COPII Member SAR1 Is Critical for the Delivery of <i>Arabidopsis</i> Xyloglucan Xylosyltransferases XXT2 and XXT5 to the Golgi Apparatus.

Plants (Basel, Switzerland)·2026
Same author

Comparative multi "omics" profiling of <i>Gossypium hirsutum</i> and <i>Gossypium barbadense</i> fibers at high temporal resolution reveals key differences in polysaccharide composition and associated glycosyltransferases.

Frontiers in plant science·2026
Same author

Different strategies of altering the cell wall to study the impact that these introduced changes have on plant resistance to pathogens.

Frontiers in plant science·2025

相关实验视频

Updated: Jul 3, 2025

Measurement of Chitinase Activity in Biological Samples
00:03

Measurement of Chitinase Activity in Biological Samples

Published on: August 22, 2019

10.3K

植物根相关酸酶:结构和功能

Samuel O Shobade1,2, Olga A Zabotina1,2, Marit Nilsen-Hamilton1,2

  • 1Ames National Laboratory, U. S. Department of Energy, Ames, IA, United States.

Frontiers in plant science
|February 16, 2024
PubMed
概括
此摘要是机器生成的。

植物和细菌基因酶的特征是它们在树根球中的作用. 植物基因酶参与对抗真菌病原体的防御,而两者都可能有助于营养物质的循环利用.

关键词:
在C端域域.它具有抗真菌活性,具有抗真菌活性.基结合域是一个基结合域.酸酸酶的使用方法酸盐的含量是多少 酸盐根系球 (Rhizosphere) 是一个根系球.

更多相关视频

Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper
06:36

Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper

Published on: February 27, 2021

3.7K
Author Spotlight: Integrating Biochemical Functions of &#946;-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction
10:26

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

676

相关实验视频

Last Updated: Jul 3, 2025

Measurement of Chitinase Activity in Biological Samples
00:03

Measurement of Chitinase Activity in Biological Samples

Published on: August 22, 2019

10.3K
Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper
06:36

Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper

Published on: February 27, 2021

3.7K
Author Spotlight: Integrating Biochemical Functions of &#946;-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction
10:26

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

676

科学领域:

  • 生物化学 生物化学
  • 植物科学 植物科学
  • 微生物学 微生物学

背景情况:

  • 酸酶降解酸,酸是真菌细胞壁和关节动物外骨架的关键组成部分.
  • 这些酶被分泌到树根球中,这是植物微生物营养交换的关键区域.
  • 了解基因酶的功能对于阐明植物微生物相互作用至关重要.

研究的目的:

  • 模拟,表达,净化和描述来自Zea mays (ZmChi19A) 和Oryza sativa (OsChi19A) 的根基基因酶和来自Chitinophaga oryzae的细菌基因酶 (CspCh18A).
  • 为了确定酶对各种基质和黑色阿斯伯菌的活性.
  • 确定特定蛋白质域 (CBD,C端域) 在酶功能和真菌生长抑制中的作用.

主要方法:

  • 酶的表达,净化和表征.
  • 使用4-MU-GlcNAc3基质进行动态分析.
  • 测定pH值和温度的最佳值.
  • 结构建模和ZmChi19A.的局部定向突变发生.
  • 用Aspergillus niger进行酶活性测定.

主要成果:

  • ZmChi19A和OsChi19A属于GH19家族;CspCh18A属于GH18家族.
  • 这三种酶对4-MU-GlcNAc3.3具有相似的表面Km值.
  • 酶显示出不同的pH值和最佳温度.
  • C终端域对溶性基质的活性至关重要,而CBD和C终端域对于抑制真菌生长至关重要.

结论:

  • 植物基因酶 (ZmChi19A,OsChi19A) 可能有助于植物防御携带基因的病原体.
  • 细菌胆酶 (CspCh18A) 和植物胆酶可能有助于根球中的营养循环.
  • 结构域在基板裂变和真菌生长抑制中起着不同的作用,突出显示了复杂的功能适应.