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metionin 源和基因型影响胚胎肠道发育,抗氧化剂,紧密结合和与的生长相关的基因表达

James K Lugata^1,2, Sawadi F Ndunguru^3,2,4, Gebrehaweria K Reda^3,2,4

¹Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.

Animal nutrition (Zhongguo xu mu shou yi xue hui)

|February 16, 2024

概括

在卵内注射 metionin (Met) 改善了的肠发育. 不同的基因型对Met有独特的反应,影响肠道抗氧化剂和生长基因表达.

科学领域:

动物科学动物科学
发展生物学发展生物学
营养生物化学营养生物化学

背景情况:

氨酸 (Met) 是一种必需的氨基酸,对家禽生长和胚胎发育至关重要.
了解Met对不同基因型肠道发育的影响对于优化家禽生产至关重要.

研究的目的:

研究DL-Met和L-Met在卵内注射对两个不同的基因型的肠道发育和健康的影响.
为了比较商业层杂交 (TETRA-SL) 和本地品种 (匈牙利色母) 在胚胎发育期间补充 metionin的反应.

主要方法:

在胚胎发育17.5日,从TETRA-SL和Hungarian Partridge彩色基因型的受精卵注射了DL-Met,L-Met,盐水或没有任何东西.
化后,对膜组织进行了分析,以确定形态参数 (膜高度,宽度,面积,密室深度) 和与抗氧化剂,紧密结合和生长因素相关的基因表达.

主要成果:

在卵子中注射的甲氨酸 (DLM和LM) 显著增强了肠道发育,与对照组相比,增加了小毛病尺寸.
与L-Met相比,在TETRA-SL基因型中注射DL-Met导致了减少密码深度和特定基因表达变化 (GST3,OCLN).
与TETRA-SL基因型相比,匈牙利色母基因型表现出IGF1,MD2,OCLN,SOD1和GST3基因的更高表达.

结论:

在卵子中,氨酸的使用对的胚胎肠道发育和功能产生了积极的影响.
的基因型对补充 metionin 的反应不同,影响关键的肠道基因.
这项研究强调了 in ovo 养在提高家禽肠道健康和性能方面的潜力,并考虑了基因型特异性.

关键词:

这是一种DL-Methionine.在卵子内注射.肠道发育肠道发育对于L-Methionine来说,这是一个很好的方法.禽类禽类禽类是一种

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