COMPASS核心子单元MpSet1和MpSwd3调节了Monascus紫色中的Monascus颜料合成
Zhongling Wu1, Hongyan Gao1, Zhenmin Liu1
1State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai, China.
Journal of basic microbiology
|February 16, 2024
概括
COMPASS 核心子单元 MpSet1 和 MpSwd3 通过建立 histone H3 lysine 4 甲基化 (H3K4me2 / 3) 对于 Monascus purpureus 的生长,发育和 Monascus 颜料 (MP) 生产至关重要. 它们还保持细胞壁的完整性.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 菌类学 菌类学是指菌类学.
背景情况:
- 基因组甲基化,特别是H3K4me,对于真核生物的基因调节和生物体发育至关重要.
- 对于COMPASS综合体在Monascus中建立H3K4me的作用仍然在很大程度上是未知的.
- 莫纳斯色素 (MP) 是一种有价值的化合物,具有多种应用.
研究的目的:
- 为了研究Monascus purpureus中COMPASS核心子单位 (MpSet1和MpSwd3) 的功能.
- 阐明它们在H3K4me2/3建立中的作用及其对生长,发育和色素生产的影响.
- 了解它们对细胞壁完整性的贡献.
主要方法:
- 在Monascus purpureus中识别和描述MpSet1和MpSwd3.
- 基因淘汰研究 (ΔMpset1, ΔMpswd3) 来评估表型变化.
- 测量Monascus颜料生产的情况.
- 高通量RNA测序和qRT-PCR用于基因表达分析.
- 对细胞壁应力敏感性的评估.
主要成果:
- 证实MpSet1和MpSwd3是COMPASS核心子单位,对于H3K4me2/3的建立至关重要.
- 这些子单元的损失导致生长,发育受损,并显著减少了Monascus色素的生产.
- 基因表达分析显示,许多基因的转录发生了变化,包括那些参与色素合成和细胞壁组件 (酸合成酶) 的基因.
- ΔMpset1 和 ΔMpswd3 菌株对细胞壁应激的敏感性增加.
结论:
- 对于Monascus purpureus中的H3K4me2/3来说,COMPASS核心子单位MpSet1和MpSwd3是至关重要的.
- 这些子单元调节关键的发育过程,包括子形成和Monascus色素生物合成.
- MpSet1和MpSwd3在维持细胞壁完整性方面发挥着作用,这表明在真菌生理学中具有更广泛的表观遗传调节作用.
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