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基因组规模代谢建模人乳寡糖类利用由Bifidobacterium longum亚种. 婴儿科的孩子们

Loreto Román¹, Felipe Melis-Arcos¹, Tomás Pröschle¹

¹Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile.

|February 16, 2024

概括

双细菌长子物种双菌长子物种使用基因组规模模型重建了人乳寡糖类 (HMO) 的婴儿代谢. 这提供了一个系统的视图,这个关键的婴儿肠道微生物如何利用母乳中发现的复杂碳水化合物.

科学领域:

微生物代谢和系统生物学
肠道微生物组研究研究
营养生物化学营养生物化学

背景情况:

双细菌长子物种双菌长子物种婴儿肠是婴儿肠道中占主导地位的有益细菌.
这种物种适应使用母乳中的复杂碳水化合物母乳寡糖化物 (HMO).
缺乏对HMO消费期间B. infantis代谢的系统层面的理解.

研究的目的:

重建Bifidobacterium longum亚种的基因组规模的代谢网络. 孩子们的孩子们.
开发特定环境的代谢模型,整合转录组数据,用于HMO利用.
提供对各种HMO的B. infantis代谢的系统视图.

主要方法:

精细的基因组规模网络重建 (iLR578) 基于现有模型,基因组注释,文献和转录基因组数据.
整合RNA-seq数据以构建特定环境的代谢模型,用于乳糖和三个不同的HMO的生长.
流量合分析以确定代谢途径的相互连接.

主要成果:

代谢重建 (iLR578) 包括578个基因,1047个反应和924个代谢物.
模型揭示了独特的HMO代谢,特别是对于fucosyl-lactose,由于其fucose部分.
在增长最大化下,高预测的乳酸盐和酸盐产量,较低的格式,乙醇和1,2-二醇.
模型预测与中央碳代谢流和表达数据有很好的一致性.
流量合分析发现了糖酸盐交换,氨酸/硫酸盐代谢和氨酸代谢之间的新联系.

结论:

开发的基因组级代谢网络和模型为研究B. infantis代谢提供了宝贵的平台.
这种系统视图增强了对B. infantis如何利用HMO等复杂碳水化合物的理解.
这些发现有助于理解这一关键的婴儿肠道微生物在营养代谢中的作用.

关键词:

这种细菌是Bifidobacterium longum subsp.infantis 的一种细菌.这是GSMM,GSMM.细菌的新陈代谢是细菌的代谢.基因组规模的代谢模型婴儿肠道微生物组在婴儿肠道微生物组

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