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在植入前胚胎发育过程中对异形切换和可移植元素表达的单细胞分析

Chaoyang Wang^1,2,3, Zhuoxing Shi³, Qingpei Huang^1,2

¹GMU-GIBH Joint School of Life Sciences, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Laboratory, Guangzhou Medical University, Guangzhou, China.

|February 16, 2024

概括

这项研究通过分析异构体表达动态,揭示了对早期胚胎发育的新见解. 研究人员发现了独特的3-prime部分转录和动态可转换元素变化,这对于理解基因调节至关重要.

科学领域:

分子生物学分子生物学
发育生物学发展生物学
基因组学就是基因组学.

背景情况:

替代拼接产生蛋白质多样性,这对发育和病原发生是必不可少的.
了解早期胚胎发生的异形动力学对于破译分子机制至关重要.
之前的研究集中在异形发现上,使得转录动态未被探索.

研究的目的:

在小鼠植入前开发过程中调查替代拼接和转录异形表达的动态.
确定涉及早期胚胎发生的新型转录类型和调控元素.
探索可移植元素在发育过程中调节基因表达中的作用.

主要方法:

从卵子细胞到胚囊细胞阶段,对100多个小鼠芽质体进行单细胞直接异形测序.
在植入前开发过程中对异形表达特征的量化.
长读序列化用于分析可转换元素 (TE) 在特定位置的表达.

主要成果:

在卵细胞和胚胎细胞中缺乏停止编码子的高积累的3-prime部分转录,可能参与母体到胚胎细胞过渡 (MZT).
在植入前开发过程中识别了3,894个具有动态表达变化的可转移元素位点.
证明这些动态TE可能调节邻近基因的表达.

结论:

早期的小鼠胚胎表现出独特的转录组特征,包括3-prime部分转录,而不是仅仅是副产品.
动态可转移元素表达在早期发育过程中在调节基因表达方面发挥着重要作用.
这项研究为管理哺乳动物植入前发育的复杂转录调节提供了新的见解.

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