化探针的通用光关闭/启动策略:硬质排斥诱导的扭曲分子内电荷转移 (sr-TICT)
Kenjiro Hanaoka1, Takayuki Ikeno2, Shimpei Iwaki2
1Graduate School of Pharmaceutical Sciences, Keio University, 1-5-30 Shibakoen, Minoto-ku, Tokyo 105-8512, Japan.
Science advances
|February 16, 2024
概括
研究人员开发了一种新的光控制策略,称为硬体排斥诱导的TICT (sr-TICT),用于设计化探针. 这种方法通过分子内扭曲加速光灭,使生物事件可视化.
科学领域:
- 化学生物学 化学生物学
- 生物物理化学 生物物理化学
- 分子成像学分子成像学
背景情况:
- 化探针是通过光变化可视化生物过程的重要工具.
- 在探头中控制光调制通常依赖于各种策略.
- 罗达胺染料是开发光传感器的广泛使用的支架.
研究的目的:
- 用时间依赖密度函数理论 (TD-DFT) 来计算分析罗达胺染料中扭曲的分子内电荷转移 (TICT) 过程.
- 设计和合成基于罗达胺的新型化探针,利用一种新的光控制策略.
- 开发一种用于可视化细胞染色体P450 3A4 (CYP3A4) 的N-脱化活性的探针.
主要方法:
- 时间依赖密度函数理论 (TD-DFT) 在罗达胺染料中对TICT的计算分析.
- 新型罗达胺衍生物的设计和合成.
- 开发一种固体排斥诱导的TICT (sr-TICT) 光灭策略.
- 应用sr-TICT探针 (2-Me PeER) 进行基于CYP3A4活动的光激活细胞分类 (FACS).
主要成果:
- 计算分析阐明了罗达胺染料中的TICT机制.
- 建立了一个新的sr-TICT策略,通过分子内扭曲显著加速光火.
- 为检测CYP3A4的N-脱化活性,开发了一种特殊的探针2-Me PeER.
- 2-Me PeER 探测器在人类诱导的多能干细胞 (hiPSC) 衍生肝细胞和肠道细胞中成功实现了基于 CYP3A4 活动的 FACS.
结论:
- sr-TICT战略提供了一种通用和有效的方法,用于控制探头设计中的光.
- 开发的探针和方法方便在相关细胞模型中研究酶活性.
- 这种方法为推进生物事件可视化和药物代谢研究提供了强大的工具.
更多相关视频
14:12Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
5.3K
13:47TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
Published on: January 29, 2015
10.8K
相关概念视频
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K