在铁氧化物纳米颗粒上直接结合和表征乳糖酶
Amanpreet K Sidhu1, Sucheta N Patil2, Vishwas B Gaikwad3
1Assistant Professor, Department of Biotechnology, Khalsa College, Amritsar, Punjab, India.
Nanotechnology
|February 16, 2024
概括
在氧化铁纳米颗粒 (IONPs) 上固定乳酶可提高其稳定性和可重复使用性. 这种具有成本效益的方法提高了各种工业应用的酶性能.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
背景情况:
- 氧化铁纳米粒子 (IONPs) 为酶结合提供了高的表面与体积比.
- 乳酶 (EC 1.10.3.2) 是一种具有广泛应用的氧化酶.
- 在IONP上的酶固定可以提高稳定性和可重复使用性,降低成本.
研究的目的:
- 通过碳胺激活,将laccase结合到合成的IONPs上.
- 用醇和聚乙烯醇 (PVA) 封闭剂稳定IONP.
- 为了描述laccase-IONP结合物的特征,并评估它们的性能.
主要方法:
- 通过热化学共降的IONPs的合成.
- 通过碳胺激活,直接将laccase与IONPs结合.
- 使用紫外光谱学,FTIR,XRD,SEM和EDX进行表征.
- 对结合效率和酶可重复使用性的评估.
主要成果:
- FTIR证实了成功的乳糖酶对IONPs的结合.
- 约束效率达到90.65% (硫糖醇) 和73.02% (PVA).
- 在20个重复使用循环后,固定化的laccase保持了~50%的活性.
结论:
- 在IONP上的Laccase固定显著提高了酶活性,稳定性和可重复使用性.
- 这种方法为降低成本和扩展应用提供了潜力.
- 基于IONP的酶固定是生物技术中一个有前途的策略.
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