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通过下一代测序来区分神经病患者中人类JC多重瘤病毒的高度致病性菌株

Eeva Auvinen¹, Anni Honkimaa¹, Pia Laine²

¹Department of Virology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

|February 16, 2024

概括

重组的JC多细胞瘤病毒 (JCPyV) 非编码控制区域 (NCCRs) 在渐进的多焦点白内障 (PML) 患者的大脑和脑脊液中很常见. 下一代测序有助于检测神经变性JCPyV,用于早期诊断.

科学领域:

病毒学病毒学
神经科学是一个神经科学.
基因组学就是基因组学.

背景情况:

简体中文简介:JC多重瘤病毒 (JCPyV) 是一种常见的人类病毒,通常无症状.
免疫性损害可以触发JCPyV的重新激活和基因组重组,特别是在非编码控制区域 (NCCR).
重组的JCPyV菌株与渐进的多焦点白血脑病 (PML) 有关.

研究的目的:

来自PML患者和其他样本类型的JCPyV菌株中的NCCR的特征.
调查不同临床和环境样本中重新排列NCCR的流行率.

主要方法:

用下一代测序 (NGS) 和生物信息学来分析NCCRs.
样本包括PML患者的脑脊液 (CSF) 和脑组织,狼患者的尿液,对照脑组织和废水.
在诊断PML样本上进行了定量PCR (qPCR).

主要成果:

在一些CSF和组织样本中发现了原型和原型类NCCR.
在PML患者样本中,重新排列的NCCR普遍存在: 8/21的CSF和14/16的大脑组织.
在C和D块中删除的特征是大多数重新排列的JCPyV菌株;在一些样本中,NCCR是不可放大的.

结论:

重组的NCCR在脑组织中占主导地位,在PML患者的CSF中很常见.
在CSF或大脑组织中敏感的NGS检测神经向性JCPyV可以帮助早期诊断.
改进的诊断可能会导致及时干预和更好的患者结果.

关键词:

诊断诊断诊断诊断在JC的多重病毒中,多重病毒有很多种.国家储备系统 (NGS)这是PML的PML.有针对性的测序.

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