相关实验视频

Updated: Jul 2, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

基于阿佐的色度和度化学传感器用于释放氧的氧

Yang Zhou¹, Jiajun Chen¹, Yunxi Cui²

¹Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education and School of Chemistry and Chemical Engineering, Hainan University, Haikou, Hainan, 570228, China.

Nitric oxide : biology and chemistry

|February 16, 2024

概括

研究人员开发了一种基于阿佐的新型供体 (Azo-D1),用于精确的氧化 (HNO) 气体释放和检测. 这种化学传感器可以实时监测生物系统中的HNO,从而推动HNO研究.

科学领域:

化学生物学化学生物学
生物化学生化学
分子成像学分子成像学

背景情况:

氧化 (HNO) 是一种具有重要的信号分子,具有多种生物作用.
由于HNO的寿命短且释放具有挑战性,因此阻碍了其研究.
现有的HNO检测方法在范围和实时应用方面有限.

研究的目的:

开发一种新的HNO捐赠器和化学传感器,用于精确的HNO释放和实时检测.
为了描述HNO释放动力学和石化计.
为了证明开发的探头用于细胞成像和HNO监测的实用性.

主要方法:

合成一种基于阿佐的HNO供体 (Azo-D1).
对HNO释放的光谱光度和度分析.
使用素化合物陷验证HNO静脉测量.
使用共聚焦显微镜进行细胞成像研究.

主要成果:

在生理条件下,Azo-D1表现出稳定的HNO释放,半衰期为~68分钟.
颜色从色到黄色的变化表明了供体分解.
在424nm的光强度增加了6.8倍,允许定量检测HNO.
在不消耗HNO源的情况下,成功实现了HNO释放的实时细胞成像.

结论:

该Azo-D1探头提供了一个可靠的平台,用于精确释放和敏感检测HNO.
这种化学传感器有助于实时监测生物环境中的HNO.
开发的Azo-D1供体为探索HNO的生物化学和生物学开辟了新的途径.

关键词:

细胞成像细胞成像提供者分子分子.光是一种光效应.氧化 (HNO) 是一种探测器探测器探测器

更多相关视频

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

相关概念视频

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...