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聚龙的cillinerve多糖类通过调节microRNA-181-181来抑制传染性胃肠炎病毒

Xueqin Duan¹, Huicong Li², Xuewen Tan²

¹College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China; Agricultural Management Department, Sichuan Xuanhan Vocational Secondary School, Xuanhan 636350, PR China.

Veterinary journal (London, England : 1997)

|February 16, 2024

概括

波利贡 (Polygonum cillinerve) 的多糖 (PCP) 通过抑制病毒复制和减少亡,有效地治疗小猪感染传染性胃肠炎病毒 (TGEV). 这项研究强调了PCP.

科学领域:

兽医病毒学兽医病毒学
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

传染性胃肠炎病毒 (TGEV) 显著影响猪健康,改变细胞微RNA (miRNA) 表达.
现在,Polygonum cillinerve多糖 (PCP) 正在研究其对TGEV的治疗潜力.

研究的目的:

评估PCP在治疗TGEV感染小猪的疗效.
阐明PCP抗病毒和抗亡作用背后的分子机制,重点关注miRNA调节.

主要方法:

在TGEV感染小猪的体内实验.
高通量测序以识别差异表达的miRNAs.
使用PCP和miR-181模仿剂/抑制剂治疗PK15细胞的体外研究.
实时PCR,电子显微镜,霍希斯特染色和西部斑点分析.

主要成果:

PCP (250μg/mL) 显著抑制了TGEV gRNA复制和N基因表达 (P < 0.01).
PCP治疗减少了病毒载量,改善了细胞形态,并减少了受感染细胞的亡.
确定了miR-181作为一个关键的miRNA,参与PCP通过Caspase依赖的线粒体通路抑制TGEV诱导的亡.

结论:

PCP显示出对小猪TGEV感染的显著治疗潜力.
通过抑制病毒复制和抑制TGEV诱导的亡,PCP发挥其作用,可能由miR-181调解.
调查结果为开发基于PCP的TGEV控制策略提供了基础.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.这是一架米R-181型飞机.多角体cillinerve多糖化物多糖化物.可传播的胃肠炎病毒.病毒复制是病毒的复制.

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