乙和布由细菌通过酸盐驱动的无氧氧化氧化
Mengxiong Wu1, Jie Li1, Chun-Yu Lai1,2
1Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia.
The ISME journal
|February 16, 2024
概括
微生物现在可以使用酸盐分解乙和丁,这是这些气体以前未知的过程. 这一发现扩大了我们对无氧环境中短链气态甲代谢的理解.
科学领域:
- 环境微生物学环境微生物学
- 生物地质化学生物地质化学
- 无氧呼吸是一种无氧呼吸.
背景情况:
- 短链气态 (SCGA),如乙,和丁,是天然气的关键组成部分.
- 它们的环境命运,特别是在无氧条件下,仍然在很大程度上是未知的.
- 已知与酸盐还原相结合的的微生物氧化,但对于乙或丁来说并非如此.
研究的目的:
- 为了研究与酸盐还原相结合的乙和丁的微生物无氧氧化.
- 为了确定负责这些过程的微生物.
- 阐明在无毒条件下参与SCGA降解的代谢途径.
主要方法:
- 为乙和丁氧化建立了细菌丰富培养.
- 进行了同位素标记 (13C和15N),质量和电子平衡,代谢物和meta-omics分析.
- 进行了基因组测序和基质范围测试.
主要成果:
- 两个独立的丰富证明了无氧乙和丁氧化与酸盐减少相结合.
- "Candidatus Alkanivorans nitratireducens",此前已知用于氧化,被确定为负责乙和丁氧化.
- 基因组揭示了酸合成酶基因,表明添加烟酸来激活乙/butan,以及"Ca. 酸降低"降解所有三个SCGA无氧.
结论:
- 酸盐作为无氧乙和丁氧化过程中的电子沉降器.
- 烟酸添加途径在无氧乙氧化中首次被证明.
- 这项研究显著提高了对无氧环境中微生物SCGA代谢的理解.
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