灵活的基因组岛屿保护在淡水和海洋Methylophilaceae
Paul Layoun1,2, Mario López-Pérez3, Jose M Haro-Moreno3
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre CAS, 37005 Ceske Budejovice, Czech Republic.
The ISME journal
|February 16, 2024
概括
在Methylophilaceae细菌中的基因组精简导致了淡水和海洋的海底鱼类血统. 尽管存在利基差异,但它们具有相似的灵活基因组岛屿和重组动态,揭示了保存的进化策略.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 甲基菌的细菌从淡水沉积物演变为海底淡水和海洋环境.
- 这种转变导致了海物种的基因组减少 (基因组简化).
- 之前的研究没有比较淡水的基因组结构和微生物多样性 ("Ca. 甲基) 和海洋 (OM43) pelagic 血统.
研究的目的:
- 为了比较两个基因组精简的海性Methylophilaceae血统的基因组结构和微生物多样性:淡水"Ca. 甲基米卢斯"和海洋OM43.3.
- 研究这些血统之间的利基区分和息地偏好.
- 分析灵活的基因组岛屿 (fGI) 和移动基因元素在它们的适应和进化中的作用.
主要方法:
- 全基因组测序和分析91'Ca. 来自欧洲湖泊的Methylopumilus'菌株和来自沿海海洋环境的12个OM43菌株.
- 比较基因组学以评估基因组结构,合成和灵活的基因组岛屿 (fGI).
- 分析元基因组数据以确定fGI的丰度和变异性.
主要成果:
- 观察到显著的利基区分,与不同的息地偏好和季节性分布"Ca. 梅西洛普米卢斯和OM43系.
- 两种谱系之间的高合成性保存,其特点是类似的位置和类型的fGI.
- 确定了三种主要的fGI,涉及菌体防御,代谢功能和营养物质获取 (,胺,血红素),含量各不相同.
- 菌株特异性基因变异与低基质度的差异生长和物种内的潜在生物地理分离有关.
- 证据表明,类似的动力学涉及移动遗传元素和重组在甜水和海洋成员的甲基类.
结论:
- 尽管有不同的生态,但基因组精简的淡水和海洋甲基因系表现出保存的基因组结构和进化动态.
- 灵活的基因组岛屿在菌株和种群层面的适应和变异中起着至关重要的作用.
- 这项研究提供了对基因组微型多样性和在甲基类植物家族内的进化趋同的见解.
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