细菌使用明显近期起源的代谢拼接路径来降解合成缓冲化合物TRIS
Johannes Holert1, Aron Borker1, Laura Lucia Nübel1
1Institute for Molecular Microbiology and Biotechnology, Microbial Biotechnology & Ecology Group, University of Münster, Münster, D-48149, Germany.
The ISME journal
|February 16, 2024
概括
科学家们发现了一种新的细菌途径,用于降解常见的缓冲剂TRIS (2-amino-2-(hydroxymethyl) propane-1,3-diol). 这种在Pseudomonas中发现的途径涉及特定的酶,并且可以在细菌之间转移,突出显示在工程环境中快速进化.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 合成缓冲剂TRIS (2-amino-2-(hydroxymethyl) propane-1,3-diol) 被广泛使用,但其生物降解途径以前是未知的.
- 工程环境可以推动新型微生物代谢途径的演变.
研究的目的:
- 为了阐明TRIS的完整细菌降解途径.
- 研究TRIS生物降解的遗传基础和进化起源.
- 为了确定这个途径的水平基因转移潜力.
主要方法:
- 从环境样本 (污水污泥,废水) 中分离和培养TRIS降解细菌.
- 基因组和转录组分析以确定涉及TRIS降解的基因.
- 不同基因表达和酶的功能特征.
- 结合转移实验,以评估水平基因转移能力.
主要成果:
- 发现了TRIS的新型细菌降解途径,涉及两个基因集群.
- 集群I编码用于TRIS氧化为2-基甲基的酶;集群II编码用于其转化为pyruvate的酶.
- 完整的途径通过结合成功转移到Pseudomonas putida KT2440.0.
- 在全球范围内,在其他细菌属 (伪,新) 中发现了非常相似的TRIS降解基因集群.
结论:
- 最近,TRIS降解通过基因招募和酶适应而演变.
- 该途径是通过横向基因转移传播的,并且分布在全球.
- 这项研究表明,微生物的快速适应和新代谢途径的出现是对人为化合物的反应.
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