Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

13.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
13.1K
Protein Networks02:26

Protein Networks

4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Families02:47

Protein Families

15.3K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key...
15.3K
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

12.9K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
12.9K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

6.4K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Glomhopper-a subfamily of DUF3504-encoding CryptonA elements in Glomeromycota.

Mobile DNA·2026
Same author

Chloroflexota in agricultural soils: current knowledge and future research directions.

Frontiers in microbiology·2026
Same author

A survey of ADP-ribosyltransferase families in the pathogenic Legionella.

BMC genomics·2025
Same author

Role of plant growth-promoting bacteria (PGPB) in enhancing phenolic compounds biosynthesis and its relevance to abiotic stress tolerance in plants: a review.

Antonie van Leeuwenhoek·2025
Same author

Searching for genes determining the APR phenotype in rye.

BMC plant biology·2025
Same author

Protein family neighborhood analyzer-ProFaNA.

PeerJ·2023

相关实验视频

Updated: Jul 2, 2025

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

6.2K

金塔罗:一种类似蛋白激酶的数据库.

Bartosz Baranowski1, Marianna Krysińska2, Marcin Gradowski3

  • 1Laboratory of Plant Pathogenesis, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

BMC research notes
|February 16, 2024
PubMed
概括
此摘要是机器生成的。

基因塔罗数据库整合了蛋白类激酶 (PKL) 蛋白家族的信息,包括新型蛋白. 这一资源有助于研究人员了解PKL蛋白质.

关键词:
这个HMM就是HMM.酸化是指酸化的方法.光转移酶的使用方法蛋白质激酶酶是一种蛋白质激酶.伪基因酶是一种伪基因酶.结构预测结构预测

更多相关视频

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

8.2K
Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

18.4K

相关实验视频

Last Updated: Jul 2, 2025

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

6.2K
Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

8.2K
Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

18.4K

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 蛋白激酶类 (PKL) 蛋白共享一个共同的3D折叠,并表现出除了酸化之外的多样化的酶活性.
  • 在生物过程中,PKL蛋白质至关重要,包括病原体的生存和癌症和神经系统疾病等人类疾病.
  • 扩大的PKL蛋白超级家族需要持续收集信息.

研究的目的:

  • 建立一个集中资源,收集和传播有关PKL蛋白家族的信息.
  • 提供关于PKL家族的全面数据,包括目前在已建立的数据库中未被认可的数据.

主要方法:

  • 开发了金塔罗数据库 (http://bioinfo.sggw.edu.pl/kintaro/).
  • 整合了70多个PKL家族的广泛注释的蛋白质序列数据.
  • 包括32个在现有域名数据库中缺少的新型PKL家族.

主要成果:

  • 金塔罗提供了一个可搜索的平台,用于PKL蛋白信息.
  • 该数据库提供了家庭描述,序列,对齐,HMM模型和3D结构的访问权限.
  • 有注释的实验结构和带有催化残留的序列标志可用.

结论:

  • 对于研究多样化的PKL蛋白超级家族的研究人员来说,Kintaro是一个宝贵的资源.
  • 该数据库有助于探索PKL蛋白的功能和进化关系.
  • 金塔罗支持正在进行的关于PKL蛋白在健康和疾病中的作用的研究.