环共生性Sacharibacteria诱导它们宿主细菌中的细胞内脂质滴滴的产生
Pu-Ting Dong1,2, Jing Tian3, Koseki J Kobayashi-Kirschvink4,5
1Department of Microbiology, The ADA Forsyth Institute, Boston, MA 02142, United States.
The ISME journal
|February 16, 2024
概括
萨卡里细菌 (TM7) 细菌与Schaalia odontolytica形成强制共生,改变宿主膜流动性并诱导保护性脂质滴. 这种相互作用凸显了微生物共生关系中的上下文依赖的好处.
科学领域:
- 微生物学 微生物学
- 微生物生态学 微生物生态学
- 交生研究 交生研究
背景情况:
- 萨卡里细菌 (原名TM7) 是来自候选细胞辐射的超小细菌,生物学知之甚少.
- 纳米synbacter lyticus TM7x是人类口腔微生物组中第一个分离的Sacharibacteria,与Schaalia odontolytica XH001.1形成一个有义务的表交.
- 这种共生允许TM7x的传播,但使宿主细菌受到压力.
研究的目的:
- 通过使用超分辨率光成像来研究Nanosynbacter lyticus TM7x和Schaalia odontolytica XH001之间的物理关联.
- 了解TM7x结合对宿主细胞膜流动性和细胞内变化的影响.
- 探索TM7x诱导的脂质滴在压力下宿主生存中的作用.
主要方法:
- 超高分辨率光成像以可视化TM7x-XH001协会.
- 无标签的拉曼光谱检测,以确认脂质滴滴的形成,并确定表型变化.
- 在各种压力条件下培养XH001.
- 生存测试用于评估脂质滴的保护作用.
主要成果:
- TM7x结合显著改变了XH001.1.的膜流动性.
- 在XH001中观察到细胞内脂质滴在与TM7x进行表交时,这是口腔细菌中的新发现.
- 拉曼光谱证实了TM7x诱导的脂质滴积累,并揭示了其他表型特征.
- 脂质滴滴积累是XH001的一般应激反应,在不利条件下增强宿主生存.
结论:
- 这项研究揭示了Sacharibacteria及其宿主之间的复杂相互作用,包括膜流动性和脂质滴滴形成的变化.
- TM7x通过脂肪滴积累来提高生存率,为其宿主Schaalia odontolytica带来潜在的好处.
- 这些发现强调了口腔微生物群中的共生关系的上下文依赖性.
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