相关实验视频

Updated: Jul 2, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Pseudomonas aeruginosa 中的 CRISPR-Cas 提供了对高菌体暴露的短暂的种群级免疫力

Bridget N J Watson¹, Loris Capria¹, Ellinor O Alseth^1,2

¹Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom.

The ISME journal

|February 16, 2024

概括

在Pseudomonas aeruginosa中,CRISPR免疫力是动态的,其稳定性取决于菌体暴露. 高累积的菌体负担可以随着时间的推移降低CRISPR的有效性,影响细菌防御策略.

科学领域:

微生物学微生物学
细菌免疫力细菌免疫力
遗传学是一个遗传学.

背景情况:

Prokaryotic CRISPR-Cas系统通过间隔器获取提供了对菌体的适应性免疫力.
之前的研究表明,Pseudomonas aeruginosa对菌体DMS3vir的CRISPR免疫力有生态驱动因素,导致菌体迅速灭绝.

研究的目的:

研究细菌群体中CRISPR间隔器获取和丧失的稳定性和动态.
确定影响CRISPR免疫力长期维持的环境条件与替代抗药策略相比.

主要方法:

进行了为期30天的细菌进化实验.
监测了CRISPR间隔器获取和丢失的动态.
评估了初始菌体剂量和再感染频率对免疫维持的影响.

主要成果:

维护CRISPR免疫力和菌体的共存取决于菌体的初始剂量和再感染率.
克里斯普尔免疫的出现和丧失与高度的间隔器多样性相关,随后由于与pilus相关的突变而下降.
累积的菌体负担是决定CRISPR在生态时间尺度上的有效性的关键因素.

结论:

克里斯普免疫不总是稳定的,可以被其他抵抗机制失去或超越.
细菌群体必须平衡CRISPR介导的免疫力与基于菌体压力的其他防御策略.
菌体负担显著影响CRISPR-Cas适应性免疫的长期疗效.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?生态动态的生态动态进化演化演化演化演化演化演化演化免疫力是一种免疫力.菌体是一种菌体.

更多相关视频

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...