通过免疫试验测量沃里可纳的稳定性和分析特征
Veni Bharti1, Benjamin Jung1,2, Sarah Lam3
1Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
The journal of applied laboratory medicine
|February 17, 2024
概括
这项研究表明,ARK免疫检测准确地测量了沃里康纳水平,与LC-MS/MS相比. 然而,在某些收集管中,沃里康纳的度随着时间的推移而下降,这影响了治疗药物监测.
科学领域:
- 临床化学 临床化学
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
背景情况:
- 沃里科纳是侵入性真菌感染的关键抗真菌剂.
- 对沃里康纳的治疗药物监测对于患者的治疗结果至关重要.
- 评估voriconazole稳定性和免疫测试性能对于准确的药物水平测量至关重要.
研究的目的:
- 在血样本中评估沃里可纳的稳定性.
- 为了确定voriconazole的ARK免疫试验的分析性能.
- 将免疫检测结果与参考LC-MS/MS方法进行比较.
主要方法:
- 在Abbott Alinity c分析仪上使用LC-MS/MS和适应的ARC免疫测试分析了患者血样本 (n=45).
- 分析性能指标包括功能灵敏度,LoB,LoD,LoQ,线性和精度被评估为免疫测试.
- 随着时间的推移,收集的样本中沃里康纳的稳定性得到了评估.
主要成果:
- 在ARK免疫测试显示高相关性 (皮尔森R=0.988) 与LC-MS/MS,与最小的偏差.
- 免疫试验显示出极好的精度和灵敏度,功能灵敏度为0.05 mg/L,LoQ为0.5 mg/L.
- 在凝分离管中采集的样本中,沃里康纳的度随着时间的推移而下降,特别是在更高度的情况下.
结论:
- 该ARK免疫测试提供可靠和可比的沃里科纳测量LC-MS/MS.
- 在凝分离管中,沃里康纳的稳定性是一个问题,可能会影响治疗药物监测.
- 免疫试验适合临床使用,提供足够的灵敏度和精度.
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