伊朗的马类百菌病:血清学,细菌学和分子分析
Maryam Amini1, Saeed Alamian2, Mahdokht Talebhemmat3
1Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
Veterinary research communications
|February 17, 2024
概括
这项研究在伊朗的看似健康的赛马中发现了布鲁塞拉抗体,表明从反动物传播的潜在跨物种传播. 分子和细菌测试证实了血清阳性马的布鲁塞拉DNA,突出了全面诊断方法的必要性.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 传染性疾病 传染性疾病
- 动物感染病原体的病原体
背景情况:
- 马类百菌病会导致马的重大健康问题,包括关节感染和.
- 兽病是一种动物传播疾病,对动物和公共健康都有影响.
- 准确诊断马群中的布鲁塞拉,对于疾病控制至关重要.
研究的目的:
- 为了调查伊朗看似健康的赛马中马类牛病的流行情况.
- 用血清学和分子方法识别影响马的布鲁塞拉物种.
- 评估不同诊断试验的可靠性,以检测马群血病.
主要方法:
- 使用孟加拉板结试验 (RBPT),血清结试验 (SAT) 和2-乙醇 (2-ME) 试验进行血清学测试.
- 从阳性和阴性马的血清样本中提取DNA.
- 来自阳性马的牛奶,血液和阴道抽样的细菌培养.
- 使用布鲁斯梯子PCR进行布鲁塞拉物种的分子鉴定.
主要成果:
- 血清测试检测到7.1% (RBPT),2.3% (SAT) 和0.2% (2-ME) 的876匹马中的布鲁塞拉抗体.
- 在44.5%的血清阳性马中确认了布鲁塞拉DNA,在26个样本中确定了布鲁塞拉堕胎,在2个样本中确定了布鲁塞拉美利丁菌.
- 从两只母马的牛奶样本中分离了Brucella melitensis biovar 1,这表明从反动物传播.
结论:
- 在伊朗,看似健康的赛马可以携带布鲁塞拉感染.
- 结合细菌学和PCR提供了更可靠的布鲁塞拉菌种的诊断. 在马匹中,不仅仅是血清学.
- 这些发现突出了布鲁塞拉从反动物传播到马匹的跨物种传播潜力,需要综合管理策略.
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