相关实验视频

Updated: Jul 2, 2025

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

SLAM-ITseq确定Nrf2通过酸路径诱导肝脏再生

Vicky W T Tan¹, Talhah M Salmi¹, Anthony P Karamalakis¹

¹Cancer Research Division, Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia; The Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, VIC 3010, Australia.

Developmental cell

|February 17, 2024

概括

肝脏的再生依赖于早期的代谢变化. 激活Nrf2抗氧化程序和酸通路 (PPP) 对肝细胞存活和损伤后核酸合成至关重要.

科学领域:

肝病学和再生医学肝病学和再生医学
分子生物学和遗传学
毒理学和环境健康

背景情况:

肝脏的再生能力非常出色,但在有毒损伤下经常失败,这是导致肝衰竭的主要原因.
肝损伤感知和再生启动的精确分子机制和时间动态尚未完全理解.

研究的目的:

阐明早期的分子和代谢事件控制肝损伤感知和再生.
研究代谢适应和抗氧化途径在肝细胞存活和肝脏修复中的作用.

主要方法:

开发一种具有肝细胞特异性 uracil phosphoribosyltransferase (UPRT) 表达的转基因斑马鱼模型.
应用SLAM-ITseq来分析肝损伤和再生期间的新生转录组.
研究Nrf2激活和酸通路 (PPP) 在肝脏再生中的必要性.

主要成果:

确定了快速的新陈代谢转变,从一个被养到一个快速的状态之前的肝脏再生.
证明了肝细胞中的核红色素2相关因子 (Nrf2) 激活对于诱导酸路径 (PPP) 是必不可少的.
表明PPP激活增强了受伤后的肝细胞存活率,PPP抑制破坏了核酸生物合成,阻止了肝脏的再生.

结论:

早期的代谢适应,包括Nrf2-介导的PPP诱导,是毒性损伤后肝脏再生的关键决定因素.
酸路径对于核酸生物合成至关重要,支持肝脏再生.
这些发现提供了关于新陈代谢重编程如何促进肝脏组织修复的基本见解.

关键词:

在G6PD中,G6PD是指G6PD.这就是NRF2的NRF2.这就是SLAM-ITseqqq.急性肝衰竭是一种严重的肝衰竭.新生转录新生转录酸酸盐路径的酸路径复兴再生是一种再生方式.斑马鱼是一种斑马鱼.

更多相关视频

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Long-patch Base Excision Repair

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are: