从养殖水产养殖到流行病学切断值的异构细菌的AMR威胁感知评估
Ranjit Kumar Nadella1, Satyen Kumar Panda2, Anuj Kumar3
1ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Quality Assurance and Management Division, Willingdon Island, Matsyapuri P.O, Cochin, Kerala 682029, India.
Journal of AOAC International
|February 17, 2024
概括
养殖场的抗生素耐药性是一个重大问题,大多数细菌对氧环素有耐药性. 这凸显了养殖池作为抗药性细菌和基因传播的潜在储备.
科学领域:
- 水产养殖微生物学 水产养殖微生物学
- 抗微生物耐药性 抗微生物耐药性
- 环境微生物学环境微生物学
背景情况:
- 抗生素耐药性是一个日益增长的全球健康风险,因在生产食品的动物中使用抗生素而加剧.
- 水产养殖环境,特别是养殖,越来越被认为是抗生素耐药性出现和传播的潜在热点.
研究的目的:
- 确定培养环境中的异质菌的流行病学切断值 (ECOFF).
- 评估这些细菌群体中对五种常见抗生素的抗生素耐药性模式.
主要方法:
- 细菌分离物从印度安得拉邦的Penaeus vannamei培养环境中获得.
- 抗生素耐药性测试使用光盘扩散方法对抗氧化环素,氨基醇,红色素,西普罗素和co-trimoxazole.
- 流行病学切断点 (ECOFF) 值使用规范抗性解释 (NRI) 分析来确定.
主要成果:
- 维布里奥病毒 spp. 和Bacillus spp. 这两种. 是主要的细菌种群.
- 观察到的最大耐药性是对氧化环素 (23.38%总体,59.4%在L6位置) 和co-trimoxazole (31.1%).
- 大多数异质型细菌分离物 (>60%) 呈现非野生类型耐药性表型,特别是对氧基环素 (90%).
结论:
- 养殖池可以作为耐药细菌的储存库,包括致病性和益生菌物种.
- 检测到非野生类型抗生素耐药表型的高患病率,特别是对氧化环素的耐药性.
- 在水产养殖环境中,细菌群体内存在耐药基因传播的重大风险.
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