相关实验视频

Updated: Jul 2, 2025

Isolation of Epithelial Cells from Human Dental Follicle

Isolation of Epithelial Cells from Human Dental Follicle

Published on: November 5, 2021

人类诱导的多能干细胞衍生牙干细胞通过表皮-介质细胞相互作用的分化

Ji-Hye Kim¹, Jihye Yang¹, Min-Gi Ki¹

¹Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.

Stem cells and development

|February 17, 2024

概括

研究人员创造了人类诱导的多能干细胞衍生牙介质干细胞 (MSCs). 这些细胞与上皮细胞结合时,显示出牙再生和自身牙修复的潜力.

科学领域:

干细胞生物学干细胞生物学
牙科再生医学牙科再生医学
发展生物学发展生物学

背景情况:

介质细胞-表皮相互作用对于牙发育至关重要.
人类诱导的多能干细胞 (hiPSCs) 为自身再生疗法提供了潜在的潜力.
之前的研究确立了hiPSC衍生的牙上皮样细胞 (EPI-iPSCs),但不是介酶干细胞 (MSCs).

研究的目的:

为了建立由hiPSC衍生的牙介质干细胞 (MSC).
与EPI-iPSCs一起将这些MSC分化为牙细胞.
评估它们对自身牙再生的潜力.

主要方法:

诱导hiPSCs通过神经形成以 PA6 stromal 细胞和神经圈培养分化为MSC.
神经介导的MSCs (NC-MSCs) 的特征是形态和标记物表达.
NC-MSCs单独培养,并与EPI-iPSCs共同培养,在牙产生诱导条件下.

主要成果:

已建立的NC-MSC表现出典型的MSC形态和表达的MSC标记.
单独使用的NC-MSC和共同培养的组都显示出牙原/矿化基因和DSPP蛋白的表达增加.
与EPI-iPSC共同培养导致较早的沉积和更高的阿梅洛基因与相关基因的表达.

结论:

由hiPSC衍生的NC-MSC具有牙分化能力.
将NC-MSC与EPI-iPSC共同培养可以提高牙的再生潜力.
由hiPSC衍生的牙干细胞代表了自牙再生的新来源.

关键词:

牙纸干细胞牙纸干细胞表皮 mesenchymal 的相互作用.人类诱导的多能干细胞干细胞.中介细胞干细胞中介细胞干细胞.神经峰的神经峰牙再生牙再生

更多相关视频

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

相关概念视频

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...