在人类胎盘样本中,微塑料积累的量化和识别使用热解气体染色学和质谱法
Marcus A Garcia1, Rui Liu1, Alex Nihart1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
概括
在所有分析的人类胎盘样本中检测到纳米和微塑料 (NMP). 这项研究提出了一种新的方法来量化人类组织中的NMP,这对于了解它们对健康的影响至关重要.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 分析化学 分析化学
背景情况:
- 全球塑料使用量正在增加,导致纳米和微塑料 (NMP) 污染.
- 核产品对人类和哺乳动物的健康构成潜在风险.
- 了解人类组织中NMP的存在对于健康评估至关重要.
研究的目的:
- 开发和验证从人体组织中提取和量化NMP的方法.
- 在人类胎盘样本中分析NMP的存在和组成.
- 建立在母胎界面进行NMP分析的定量方法.
主要方法:
- 开发了通过肥和超离心法提取组织.
- 使用热解气色谱和质谱法 (Py-GC-MS) 来识别和量化NMP.
- 用光显微镜和富里埃变换红外光谱 (FTIR) 进行基准测试.
主要成果:
- 在分析的所有62个胎盘样本中,Py-GC-MS检测到了微塑料.
- NMP度在6.5至685微克/克之间,平均为126.8微克/克.
- 聚乙烯是最丰富的聚合物 (54%),其次是聚乙烯和尼龙 (约. 每个人10%).
结论:
- 开发的Py-GC-MS方法提供了对人类组织中NMPs的无偏见的定量分析.
- 在人类的胎盘组织中,NMPs无处不在.
- 这种方法对于未来研究NMP对怀孕结果的影响至关重要.
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