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来自牛乳腺免疫球蛋白G的新型多目标ACE抑制:细胞运输,调节内皮功能障碍的有效性,以及网络药理学研究

Chu-Li Wu¹, Zi-Fu Ni¹, Xiao-Yan Kuang¹

¹Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, Guangdong China.

Journal of agricultural and food chemistry

|February 17, 2024

概括

新型血管素转化酶抑制剂 (ACEI) 从牛乳粉免疫球蛋白G (IgG) 中被确定. 这些体显示出强烈的ACEI活性,良好的稳定性和多目标效应,有利于内皮功能障碍.

科学领域:

生物化学生物化学
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

ангиотензин转化酶 (ACE) 在血压调节中起着至关重要的作用.
高血压和内皮功能障碍是严重的心血管健康问题.
牛乳腺免疫球蛋白G (IgG) 是生物活性的潜在来源.

研究的目的:

选和识别来自牛乳粉IgG的新型ACE抑制剂 (ACEI) .
评估这些的ACEI活性,吸收率,稳定性和多目标效应.
调查已识别的在改善内皮功能障碍方面的潜力.

主要方法:

采用了传统的实验室方法,生物信息学和细胞模型.
使用纳米-UPLC-MS/MS.分析了IgG的纯化蛋白酶解液.
进行了合成,体外ACEI活性测定,模拟胃肠道消化,Caco-2细胞单层模型,H2O2诱导内皮功能障碍模型 (HUVECs),网络药理学和RT-qPCR.

主要成果:

确定了8种ACEI,其中SFYPDY,TSFYPDY,FSWF,WYQQVPGSGL和GVHTFP显示出显著的ACEI活性.
SFYPDY,FSWF和WYQQVPGSGL显示出更高的吸收率和ACEI强度.
这三种在模拟消化过程中表现出稳定性,并通过降低亡和ROS,并通过上调NOS3mRNA表达来有效调节内皮功能障碍.

结论:

SFYPDY,FSWF和WYQQVPGSGL被确定为具有潜在治疗应用的新型多目标ACEI.
这些由牛乳母乳IgG衍生的在治疗高血压和内皮功能障碍方面表现有前途.
这项研究强调了结合实验室和计算方法来发现生物活性的有效性.

关键词:

这是一种ACE抑制性.在Caco-2单层中.生物信息学是一种生物信息学.内皮功能障碍模型的模型网络药理学网络药理学

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