在尿病原性大肠杆菌 (Escherichia coli) 中对氏素耐药性的细菌学和分子研究
Ali Attaallah Ibrahim1,2, Rana Kadhim Mohammed3
1Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq. ali.ata@sc.uobaghdad.edu.iq.
概括
这项研究发现,来自伊拉克的尿病原菌大肠杆菌 (UPEC) 具有高抗生素耐药性. 斯福米显示出有效性,但发现了像A3和A4这样的耐药基因,突出显示出需要新的诊断和治疗方法.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 传染性疾病 传染性疾病
背景情况:
- 泌尿病原性大肠杆菌 (UPEC) 的抗生素耐药性构成了全球健康的重大威胁.
- 了解抵抗机制对于开发有效的治疗和诊断至关重要.
研究的目的:
- 研究来自伊拉克巴格达的UPEC隔离物中的抗生素耐药性模式和菌素耐药性基因.
- 确定UPEC中菌素耐药性的遗传基础.
主要方法:
- 使用形态,生化和遗传识别方法对250个尿样进行分析.
- 抗生素敏感性测试和最低抑制度 (MIC) 和最低杀菌度 (MBC) 的确定.
- 聚合酶链反应 (PCR) 在基因组DNA和等离子体DNA中检测菌素耐药基因 (murA,glpT,cyaA,fosA3,fosA4).
主要成果:
- 大肠杆菌是最常见的细菌 (63.6%),其中99.3%的分离物表现出多药性耐药性 (MDR).
- 斯福米辛对UPEC表现出抗菌活性,MICs在512-1024μg/mL之间,MBC在2048μg/mL之间.
- 在基因组DNA中普遍检测到murA,glpT和cyaA基因,而分别在8.6%和5%的分离物中发现了fosA3和fosA4. 基于等离子体的检测显示,A3和A4的频率较低.
结论:
- 伊拉克的尿病原菌大肠杆菌呈现出抗生素耐药性的高且不断增加的患病率.
- 素仍然是一个可行的选择,但抗药性基因的存在需要进一步调查.
- 该研究提供了关于UPEC中菌素耐药性的遗传基础的见解,有助于开发新的策略.
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