相关实验视频

Updated: Jul 2, 2025

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

阿拉比多普西斯塔利亚娜的宿主反应与真菌内细胞的相互作用涉及微RNAs

Anand Mishra¹, Pooja Yadav^1,2, Kunal Singh^3,4

¹Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur, 176061, India.

Molecular biotechnology

|February 17, 2024

概括

这项研究揭示了microRNAs (miRNAs) 在Arabidopsis thaliana与内源性真菌的相互作用中发挥关键作用. 具体来说,ath-miRNA398b被显著调节,影响了植物和真菌的共生.

科学领域:

植物与微生物的相互作用
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

植物与真菌的相互作用是复杂的,并未完全理解.
真菌内细胞和地殖民植物,但机制尚不清楚.
小RNAs (miRNAs) 调节生物相互作用,但它们在植物-真菌内分植物中的作用尚不清楚.

研究的目的:

在内生真菌殖民期间,在Arabidopsis thaliana中识别差异表达的miRNA.
阐明miRNAs在调节植物和真菌相互作用中的作用.

主要方法:

利用了来自Arabidopsis thaliana的基因表达大盘的转录表达数据.
使用生物信息学工具 (psRNAtarget,C-mii,pmiREN,TarDB) 预测了miRNA及其目标基因.
进行了蛋白质-蛋白质相互作用分析,并使用定量实时PCR验证了基因/miRNA表达.

主要成果:

鉴定了15种差异表达的miRNA,具有显著的ath-miRNA398b调节.
预测了这些miRNA的357个点基因.
验证了ath-miRNA398b和目标基因 (AtSOD2,AtBXL2,AtRCA) 的表达,识别了关键的枢纽蛋白.

结论:

微RNAs在调节Arabidopsis thaliana-endophytic真菌相互作用方面发挥着重要作用.
ath-miRNA398b及其向基因在这种共生关系中至关重要.

关键词:

微生物内生菌是微生物内生菌.非致病性植物微生物相互作用.超氧化物脱酶的作用微RNA159bbb是什么意思微型RNA3988可以使用.

更多相关视频

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Published on: July 22, 2017

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Published on: July 2, 2018

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...