在经过重复致命选择的突变大肠杆菌实验群体中出现多位元突变者基因型
Mitra Maria Elgrail1,2, Kathleen Sprouffske3, Jude O Dartey1
1Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, United States.
Journal of evolutionary biology
|February 17, 2024
概括
突变基因对异性种群的突变率有所增加. 这项研究经验性地测试并证实了第二个突变分子的等位基因可以出现并固定,即使在已经含有突变分子的群体中.
科学领域:
- 进化生物学是进化的生物学.
- 微生物遗传学微生物遗传学
背景情况:
- 突变异位基因,赋予更高的突变率,自发出现和固定在无性人群.
- 理论预测,在一个突变者群体中,第二个突变者也可以出现并车固定.
研究的目的:
- 实证地测试埃舍里希亚大肠杆菌中重复突变体上车的假设.
- 在抗生素选择过程中调查第二个突变异基因的出现和固定.
主要方法:
- 暴露了一种突变的L- Escherichia coli菌株 (100倍野生型突变率) 进行连续的抗生素选择.
- 进行DNA测序,以识别终点种群中的突变.
- 使用等离子体补充试验来确认新突变的因果关系.
主要成果:
- 在终点种群中观察到高突变率和多位元突变者的丰富.
- 在两种实验复制品中确定了相同的自发1-bp插入mutT基因.
- 证实了突变T突变在增加突变率中的因果作用.
结论:
- 数据支持了重复突变者车的假设,mutT突变在人群中固定.
- 抗生素选择性强度的顺序影响了多位元突变体的丰富.
- 突变率的升级对抗生素耐药性,宿主-病原体相互作用和致癌产生影响.
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