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禽类致病性大肠杆菌Escherichia coli T6SS效应蛋白Hcp2a通过DF-1细胞与LETM1蛋白相互作用引起线粒体功能障碍

Liting Lu¹, Zhao Qi¹, Zhe Chen¹

¹Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China; Anhui Province Engineering Laboratory for Animal Food Quality and Bio-Safety, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.

Poultry science

|February 17, 2024

概括

禽类致病性大肠杆菌 (E. coli) 是一种致病性大肠杆菌.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

鸟类致病性大肠杆菌 (APEC) 中的VI型分泌系统 (T6SS) 将效应蛋白传递到宿主真核细胞中.
线粒体是已知的细菌病原体的目标,但APEC T6SS因子对线粒体的影响的具体机制尚不清楚.
血清素共同调节蛋白 (Hcp) 是APEC中一个关键的T6SS标记物和效应蛋白.

研究的目的:

研究APEC T6SS效应蛋白Hcp2a对真核宿主细胞 (DF-1细胞) 中的线粒体的影响.
为了阐明Hcp2a诱导的线粒体功能障碍背后的分子机制.
在DF-1细胞内识别与Hcp2a相互作用的宿主蛋白.

主要方法:

用纯化的Hcp2a蛋白对待DF-1细胞.
通过测量膜潜力,细胞内 (Ca2+) 和活性氧物种 (ROS) 水平来评估线粒体功能.
进行了蛋白质-蛋白质相互作用分析,包括对现有数据集的重新分析和分子对接.
确定了Hcp2a的亚细胞局部.

主要成果:

暴露DF-1细胞Hcp2a导致线粒体膜潜力下降,Ca2+度增加,ROS水平升高,表明线粒体功能障碍.
生物信息分析确定了线粒体蛋白质Leucine zipper EF-hand-containing transmembrane protein 1 (LETM1),作为Hcp2a的一个潜在的相互作用伙伴.
分子对接证实了Hcp2a和LETM1.1之间有利的结合相互作用.
亚细胞局部化研究证实Hcp2a在DF-1细胞的线粒体中积聚.

结论:

APEC Hcp2a 效应蛋白诱导DF-1 细胞中的线粒体功能障碍.
Hcp2a可能与线粒体蛋白 LETM1 相互作用,导致线粒体功能障碍,并促进 Hcp2a 局部化到线粒体.

关键词:

一个DF-1细胞.在 Hcpp 的情况下,Hcpp 禽类致病性大肠杆菌 (Escherichia coli) 是一种致病性大肠杆菌.线粒体功能障碍线粒体功能障碍第六类分泌系统.

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